Morphological studies of the canine pituitary luteinizing hormone (LH) cells after long-term treatment with the LH-releasing hormone (LHRH) agonist [D-Trp6]LHRH ethylamide.
Morphological studies of the canine pituitary luteinizing hormone (LH) cells after long-term treatment with the LH-releasing hormone (LHRH) agonist [D-Trp6]LHRH ethylamide.
复制标题
使用 LH 释放激素 (LHRH) 激动剂 [D-Trp6]LHRH 乙酰胺长期治疗后的犬垂体黄体生成素 (LH) 细胞的形态学研究。
作者：
D. Dubé;G. Pelletier;F. Labrie
由于LHRH激动剂(LHRH-A)治疗引起的黄体生成素分泌的变化与人和狗相似，我们研究了慢性给予LHRH-A，[D-Trp6]LHRH乙胺后，犬黄体生成素细胞的细胞学变化。光镜下，黄体生成素细胞在腺体内分布均匀，但注射50微克LHRH-A后1个月细胞表面积减少10%，4个月细胞表面积减少23%。在电子显微镜水平上，对照动物的促性腺激素细胞的特征是存在两种类型的分泌颗粒和粗面内质网扩张。黄体生成素的免疫染色仅定位于分泌颗粒。LHRH-A可引起胞浆内糖原的出现。停止治疗4个月后，促性腺激素细胞显示出正常的细胞学外观，从而表明长期使用LHRH-A引起的形态变化完全可逆。
Since treatment with LHRH agonists (LHRH-A) cause changes in LH secretion which are similar in both man and dog, we have studied cytological changes occurring in canine LH cells after chronic administration of an LHRH-A, [D-Trp6] LHRH ethylamide. At the light microscopic level, LH cells were distributed evenly in the gland, but showed a decrease in cell surface area of 10% after 1 month and 23% after 4 months of daily injection of 50 micrograms LHRH-A. At the electron microscopic level, the gonadotrophs from control animals were characterized by the presence of two types of secretory granules and a dilatation of the rough endoplasmic reticulum. Immunostaining for LH was localized only to secretory granules. LHRH-A administration caused the appearance of glycogen in the cytoplasm. Four months after cessation of the treatment, the gonadotrophs showed a normal cytological appearance, thus indicating full reversibility of the morphological changes induced by long-term treatment with an LHRH-A.
正常男性中有效的促性腺激素释放激素激动剂对睾丸类固醇生成和精子发生的可逆抑制：一种开发男性避孕药的方法。
DOI：
10.1056/nejm198109173051203
发表时间：
1981
期刊：
The New England journal of medicine
影响因子：
--
作者：
Linde,R;Doelle,GC;Alexander,N;Kirchner,F;Vale,W;Rivier,J;Rabin,D
通讯作者：
Rabin,D
黄体生成素释放激素激动剂降低人体黄体生成素的生物活性并改变其色谱行为。
DOI：
10.1172/jci111200
发表时间：
1984
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Evans,RM;Doelle,GC;Lindner,J;Bradley,V;Rabin,D
通讯作者：
Rabin,D
亚基对黄体生成素/人绒毛膜促性腺激素重组体功能的贡献。
影响因子：
4.8
作者：
Strickland,TW;Puett,D
通讯作者：
Puett,D