The role of iron in the growth of human leukemic cell lines
The role of iron in the growth of human leukemic cell lines
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铁在人类白血病细胞系生长中的作用
DOI：
10.1002/jcp.1041210131
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发表时间：
1984
影响因子：
5.6
通讯作者：
J. Breton‐Gorius
中科院分区：
文献类型：
--
作者：
M. Titeux;U. Testa;F. Louache;P. Thomopoulos;H. Rochant;J. Breton‐Gorius
本文研究了三种人白血病细胞系（K562，HEL，U937）在无血清条件下的生长要求。为了在无血清培养基中生长，细胞需要胰岛素、转铁蛋白和白蛋白。两种高水溶性铁盐，柠檬酸铁铵和硫酸铁铵，可以完全取代转铁蛋白，以支持这些细胞系的生长。当有丝分裂原刺激的淋巴细胞在无血清培养基中生长时，获得了类似的结果。含铁化合物，如氯化血红素或血红蛋白，也能够取代转铁蛋白。使用42/6单克隆抗体的实验强烈表明，游离铁盐通过完全独立于转铁蛋白受体的机制被细胞摄取。
The growth requirements of three human leukemic cell lines (K 562, HEL, U937) have been studied in the absence of serum. For growth in serum‐free medium, the cells require insulin, transferrin, and albumin. Two highly watersoluble iron salts, ferric ammonium citrate and ferric ammonium sulfate, may completely replace transferrin for supporting the growth of these cell lines. Similar results were obtained when mitogen‐stimulated lymphocytes were grown in serum‐free media. Iron containing compounds, such as hemin or hemoglobin, were also able to replace transferrin. Experiments using 42/6 monoclonal antibody strongly suggest that free‐iron salts are taken up by the cells by a mechanism that is completely independent from transferrin receptors.
影响因子：
56.9
作者：
MARTIN, P;PAPAYANNOPOULOU, T
通讯作者：
PAPAYANNOPOULOU, T
转铁蛋白受体单克隆抗体抑制人淋巴细胞增殖。
影响因子：
20.3
作者：
Mendelsohn,J;Trowbridge,I;Castagnola,J
通讯作者：
Castagnola,J