The Cbk1-Ace2 axis guides Candida albicans from yeast to hyphae and back again.
The Cbk1-Ace2 axis guides Candida albicans from yeast to hyphae and back again.
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Cbk 1-Ace 2轴引导白色念珠菌从酵母菌到菌丝，然后再返回。
DOI：
10.1007/s00294-020-01152-1
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发表时间：
2021-06
期刊：
影响因子：
2.5
通讯作者：
Krysan DJ
中科院分区：
文献类型：
--
作者：
Wakade RS;Krysan DJ
自S.在酿酒酵母中，Ace 2和形态发生（RAM）途径的调节已经在多种模式和病原真菌中研究了近20年。在致病真菌中，RAM途径通过仍有待详细定义的机制执行许多功能。最近，我们报道了Cbk 1介导的转录因子Ace 2的磷酸化作用抑制了白念珠菌的菌丝-酵母转化。相对于酵母到菌丝的转变，这种转变还没有得到充分研究。近顶端菌丝细胞室被逮捕在G1期，直到点，在此出现的侧酵母。在这里，我们讨论这个模型，并报告新的数据表明，第二个G1相关蛋白，有丝分裂出口调节Amn 1。In S.在酿酒酵母二倍体细胞中，Amn 1通过负反馈环在基因表达水平和蛋白质水平上通过靶向Ace 2降解来负调节Ace 2。In C.在白念珠菌中，Amn 1和Ace 2也在基因表达水平上形成反馈环。AMN 1的缺失降低了成熟菌丝中相对于野生型的侧向酵母形成，并且与PES 1（侧向酵母形成的正调节剂）的表达降低相关。这些数据表明，有丝分裂出口的调节在决定C.白色念珠菌。我们还提出了一个综合模型的相互作用之间的Cbk 1-Ace 2轴和其他菌丝阶段的监管机构的过程中，rechenation和过渡回酵母。
Since its description in S. cerevisiae, the Regulation of Ace2 and Morphogenesis (RAM) pathway has been studied for nearly 20 years in multiple model and pathogenic fungi. In pathogenic fungi, the RAM pathway carries out many functions through mechanisms that remain to be defined in detail. Recently, we reported that Cbk1-mediated phosphorylation of the transcription factor Ace2 functions to repress the hyphae-to-yeast transition in Candida albicans. This transition is understudied relative to the yeast-to-hyphae transition. Subapical hyphal cell compartments are arrested in G1 until the point at which lateral yeast emerge. Here, we discuss this model and report new data indicating that a second G1 associated protein, the mitotic exit regulator Amn1. In S. cerevisiae diploid cells, Amn1 negatively regulates Ace2 at both the gene expression level through a negative feedback loop and at the protein level by targeting Ace2 for degradation. In C. albicans, Amn1 and Ace2 also form a feedback loop at the level of gene expression. Deletion of AMN1 decreases lateral yeast formation relative to wild type in maturing hyphae and is associated with decreased expression of PES1, a positive regulator of lateral yeast formation. These data indicate that the regulation of mitotic exit plays a role in determining the timing of lateral yeast emergence from hyphae in C. albicans. We also propose an integrated model for the interplay between the Cbk1-Ace2 axis and other hyphal stage regulators during the process of filamentation and transition back to yeast.
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