Levels of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (APE1, APEX, Ref-1) are associated with the intrinsic radiosensitivity of cervical cancers.
Levels of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (APE1, APEX, Ref-1) are associated with the intrinsic radiosensitivity of cervical cancers.
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DNA修复酶的水平人类肾上腺素/阿哌丁丁核酸内切酶（APE1，APEX，REF-1）与宫颈癌的内在放射敏性有关。
DOI：
10.1038/bjc.1998.641
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发表时间：
1998-11
影响因子：
8.8
通讯作者：
Margison, GP
中科院分区：
文献类型：
--
作者：
Herring, CJ;West, CML;Wilks, DP;Davidson, SE;Hunter, RD;Berry, P;Forster, G;MacKinnon, J;Rafferty, JA;Elder, RH;Hendry, JH;Margison, GP
研究了人宫颈肿瘤固有放射敏感性与DNA修复酶人无尿嘧啶/无嘧啶核酸内切酶（HAP1）表达的关系。用软琼脂法测定肿瘤活检中克隆原细胞在2 Gy （SF2）下的存活分数的放射敏感性。重组HAP1兔抗血清对福尔马林固定石蜡包埋肿瘤切片进行染色，检测HAP1的表达水平。这两项测量都是在预处理活检材料上获得的。所有25个肿瘤均显示HAP1阳性染色，但肿瘤内部和肿瘤之间的表达水平存在异质性。肿瘤内和肿瘤间异质性的平均变异系数分别为62%和82%。HAP1表达水平与SF2呈正相关（r = 0.60, P = 0.002）。因此，本研究表明宫颈癌内在放射敏感性与DNA修复酶的表达之间存在一定的关系。结果表明，这种类型的方法可能有助于肿瘤放射敏感性的快速预测测试的发展。
A study was made of the relationship between the intrinsic radiosensitivity of human cervical tumours and the expression of the DNA repair enzyme human apurinic/apyrimidinic endonuclease (HAP1). The radiosensitivity of clonogenic cells in tumour biopsies was measured as surviving fraction at 2 Gy (SF2) using a soft agar assay. HAP1 expression levels were determined after staining of formalin-fixed paraffin-embedded tumour sections with a rabbit antiserum raised against recombinant HAP1. Both measurements were obtained on pretreatment biopsy material. All 25 tumours examined showed positive staining for HAP1, but there was heterogeneity in the level of expression both within and between tumours. The average coefficients of variation for intra- and intertumour heterogeneity were 62% and 82% respectively. There was a moderate but significant positive correlation between the levels of HAP1 expression and SF2 (r = 0.60, P = 0.002). Hence, this study shows that there is some relationship between intrinsic radiosensitivity and expression of a DNA repair enzyme in cervical carcinomas. The results suggest that this type of approach may be useful in the development of rapid predictive tests of tumour radiosensitivity.
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内在放射敏性作为对子宫颈癌放射疗法的患者反应的预后因素的独立性。
影响因子：
8.8
作者：
West, C M;Davidson, S E;Roberts, S A;Hunter, R D
通讯作者：
Hunter, R D
DOI：
10.1016/0167-8817(84)90066-x
发表时间：
1984-01-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
LABELLE, M;LINN, S
通讯作者：
LINN, S
影响因子：
10.5
作者：
Jayaraman, L;Murthy, KGK;Prives, C
通讯作者：
Prives, C
影响因子：
14.9
作者：
CHEN, DS;HERMAN, T;DEMPLE, B
通讯作者：
DEMPLE, B
DOI：
10.1073/pnas.92.8.3171
发表时间：
1995-04-11
影响因子：
11.1
作者：
PETERSON, SR;KURIMASA, A;CHEN, DJ
通讯作者：
CHEN, DJ