Preventive and therapeutic effects of nitrite supplementation in experimental inflammatory bowel disease.
Preventive and therapeutic effects of nitrite supplementation in experimental inflammatory bowel disease.
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DOI：
10.1016/j.redox.2013.12.012
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发表时间：
2014
期刊：
影响因子：
11.4
通讯作者：
Borniquel, Sara
中科院分区：
文献类型：
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作者：
Jadert, Cecilia;Phillipson, Mia;Holm, Lena;Lundberg, Jono O.;Borniquel, Sara
无机硝酸盐和亚硝酸盐已成为胃肠道中一氧化氮（NO）生成的替代底物，并已显示出对药物性胃损伤的保护作用。目的：探讨硝酸盐和亚硝酸盐对实验性结肠炎模型的预防和治疗作用。用葡聚糖硫酸钠（DSS）诱导小鼠结肠炎，同时在饮水中给予亚硝酸盐（1mm）或硝酸盐（10mm） 7天。还研究了一种治疗方法，即在dss诱导的结肠炎后3天开始亚硝酸盐治疗。评估临床和炎症指标，并在体内测量结肠粘液厚度。用结肠上皮细胞评价亚硝酸盐对伤口愈合的影响。同时给予DSS和亚硝酸盐（1 mM）可通过降低疾病活动指数评分（DAI）和增加结肠长度来减轻炎症，而硝酸盐（10 mM）仅降低DAI评分。亚硝酸盐还通过降低结肠iNOS表达和改善组织病理学来改善已建立的结肠炎症。亚硝酸盐完全阻止了dss引起的结肠黏液厚度下降。此外，DSS处理降低了杯状细胞的丰度，而添加亚硝酸盐则增加了杯状细胞的丰度。对结肠上皮细胞的进一步研究显示，亚硝酸盐对伤口愈合有no依赖性的改善。亚硝酸盐对结肠炎症具有预防和治疗双重作用。其保护作用包括保存完整的黏液层和调节上皮细胞的恢复。无机硝酸盐和亚硝酸盐可减轻dss引起的结肠炎。饮食中的亚硝酸盐对已经形成的结肠炎症有治疗作用。膳食亚硝酸盐可防止dss引起的结肠黏液层变薄。亚硝酸盐促进结肠上皮细胞愈合。
Inorganic nitrate and nitrite have emerged as alternative substrates for nitric oxide (NO) generation in the gastrointestinal tract, and have shown to be protective against drug-induced gastric injury. The aim of this study was to investigate the preventive and therapeutic effects of nitrate and nitrite in a model of experimental colitis. Colitis was induced in mice by administrating dextran sulfate sodium (DSS) with concurrent administration of nitrite (1 mM) or nitrate (10 mM) in the drinking water for 7 days. A therapeutic approach was also investigated by initiating nitrite treatment 3 days after DSS-induced colitis. Clinical and inflammatory markers were assessed and the colonic mucus thickness was measured in vivo. The effect of nitrite on wound healing was evaluated using colon epithelial cells. Concurrent administration of DSS and nitrite (1 mM) alleviated inflammation as determined by reduced disease activity index score (DAI) and increased colon length, while nitrate (10 mM) only reduced the DAI-score. Nitrite also displayed therapeutic effects by ameliorating established colonic inflammation with reduced colonic expression of iNOS and improving histopathology. DSS-induced decrease in colonic mucus thickness was completely prevented by nitrite administration. In addition, goblet cell abundance was lower by DSS treatment, but was increased by addition of nitrite. Further studies using colon epithelial cells revealed an NO-dependent improvement in wound healing with nitrite administration. Nitrite exerts both preventive and therapeutic effects in colonic inflammation. The protective effects involve preservation of an intact adherent mucus layer and regulation of epithelial cell restitution. Inorganic nitrate and nitrite alleviate DSS-induced colitis. Dietary nitrite has therapeutic effects in already established colonic inflammation. DSS-induced thinning of the colonic mucus layer is prevented by dietary nitrite. Nitrite promotes healing of colon epithelial cells.
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影响因子：
6.7
作者：
Bergstrom KS;Kissoon-Singh V;Gibson DL;Ma C;Montero M;Sham HP;Ryz N;Huang T;Velcich A;Finlay BB;Chadee K;Vallance BA
通讯作者：
Vallance BA
DOI：
10.1152/ajpgi.2001.280.5.g922
发表时间：
2001-05-01
影响因子：
4.5
作者：
Atuma, C;Strugala, V;Holm, L
通讯作者：
Holm, L
影响因子：
8.8
作者：
Björne, H;Govoni, M;Weitzberg, E
通讯作者：
Weitzberg, E
影响因子：
10.8
作者：
Carlstrom, Mattias;Persson, A. Erik G.;Lundberg, Jon O.
通讯作者：
Lundberg, Jon O.
影响因子：
3.3
作者：
通讯作者：
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