Regulation of gustatory physiology and appetitive behavior by the Drosophila circadian clock.
Regulation of gustatory physiology and appetitive behavior by the Drosophila circadian clock.
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DOI：
10.1016/j.cub.2009.12.055
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发表时间：
2010-02-23
期刊：
影响因子：
--
通讯作者：
Hardin PE
中科院分区：
文献类型：
--
作者：
Chatterjee A;Tanoue S;Houl JH;Hardin PE
化学感受过程的昼夜节律调节在动物中很常见，但关于昼夜节律钟如何控制化学感受系统或化学感受系统功能中节律的后果知之甚少。味觉是一个主要的化学感受门，用来决定动物是否会进食，果蝇的主要味觉器官，吻，拥有自主的昼夜节律振荡器。在这里，我们研究味觉生理学，味觉诱发的食欲行为，和食物摄取，以了解果蝇味觉系统的时钟依赖性调节。在这里，我们报告，从标签味觉感受器神经元（GRNs）的吸引力和厌恶的tastants的单单位反应显示昼夜节律的尖峰幅度，频率和持续时间跨越不同类别的味觉感受器。电生理反应的节律与长鼻伸展反射（PrER）的行为节律平行。GRNs中的分子振荡器对于味觉反应中的节律是必要且充分的，并且驱动G蛋白偶联受体激酶2（GPRK2）表达中的节律，所述G蛋白偶联受体激酶2（GPRK2）表达介导味觉敏感性中的节律。消除某些GRNs中的时钟功能会增加进食和运动活动，模仿饥饿反应。GRNs中的昼夜节律钟控制神经元的输出，并驱动味觉反应中的行为节律，这些反应在一天中果蝇进食最多的时候达到峰值。我们的研究结果表明，GPRK2水平的振荡驱动味觉生理和行为的节奏，GRN时钟抑制进食。在味觉系统的组织和喂养行为在苍蝇和哺乳动物的相似性，以及在人类的味觉敏感性的昼夜变化，表明我们的研究结果是相关的人类的情况。
Circadian regulation of chemosensory processes is common in animals, but little is known about how circadian clocks control chemosensory systems or the consequences of rhythms in chemosensory system function. Taste is a major chemosensory gate used to decide whether or not an animal will eat, and the main taste organ in Drosophila, the proboscis, harbors autonomous circadian oscillators. Here we examine gustatory physiology, tastant-evoked appetitive behavior, and food ingestion to understand clock-dependent regulation of the Drosophila gustatory system. Here we report that single-unit responses from labellar gustatory receptor neurons (GRNs) to attractive and aversive tastants show diurnal and circadian rhythms in spike amplitude, frequency and duration across different classes of gustatory sensilla. Rhythms in electrophysiological responses parallel behavioral rhythms in proboscis extension reflex (PrER). Molecular oscillators in GRNs are necessary and sufficient for rhythms in gustatory responses, and drive rhythms in G protein coupled receptor kinase 2 (GPRK2) expression that mediate rhythms in taste-sensitivity. Eliminating clock function in certain GRNs increases feeding and locomotor activity, mimicking a starvation response. Circadian clocks in GRNs control neuronal output and drive behavioral rhythms in taste responses that peak at a time of day when feeding is maximal in flies. Our results argue that oscillations in GPRK2 levels drive rhythms in gustatory physiology and behavior, and that GRN clocks repress feeding. The similarity in gustatory system organization and feeding behavior in flies and mammals, and diurnal changes in taste sensitivity in humans, suggest that our results are relevant to the situation in humans.
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DOI：
10.1073/pnas.0702726104
发表时间：
2007-05-15
影响因子：
11.1
作者：
Ja, William W.;Carvalho, Gil B.;Benzer, Seymour
通讯作者：
Benzer, Seymour
影响因子：
16.2
作者：
Marella, S;Fischler, W;Scott, K
通讯作者：
Scott, K
DOI：
10.1073/pnas.83.21.8410
发表时间：
1986-11-01
影响因子：
11.1
作者：
HOCHNER, B;KLEIN, M;KANDEL, ER
通讯作者：
KANDEL, ER
影响因子：
5.3
作者：
Granados-Fuentes, Daniel;Tseng, Alan;Herzog, Erik D.
通讯作者：
Herzog, Erik D.
影响因子：
9.2
作者：
Krishnan, Parthasarathy;Chatterjee, Abhishek;Hardin, Paul E.
通讯作者：
Hardin, Paul E.