Demonstration of a multi-technique approach to assess glacial microbial populations in the field
Demonstration of a multi-technique approach to assess glacial microbial populations in the field
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演示现场评估冰川微生物种群的多种技术方法
DOI：
10.1017/jog.2016.23
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发表时间：
2016
影响因子：
3.4
通讯作者：
BARNETT M
中科院分区：
文献类型：
--
作者：
BARNETT M
The ability to perform microbial detection and characterization in-field at extreme environments, rather than on returned samples, has the potential to improve the efficiency, relevance and quantity of data from field campaigns. To date, few examples of this approach have been reported. Therefore, we demonstrate that the approach is feasible in subglacial environments by deploying four techniques for microbial detection: real-time polymerase chain reaction; microscopic fluorescence cell counts, adenosine triphosphate bioluminescence assay and recombinant Factor C assay (to detect lipopolysaccharide). Each technique was applied to 12 subglacial ice samples, 12 meltwater samples and two snow samples from Engabreen, Northern Norway. Using this multi-technique approach, the detected biomarker levels were as expected, being highest in debris-rich subglacial ice, moderate in glacial meltwater and low in clean ice (debris-poor) and snow. Principal component analysis was applied to the resulting dataset and could be performed in-field to rapidly aid the allocation of resources for further sample analysis. We anticipate that in-field data collection will allow for multiple rounds of sampling, analysis, interpretation and refinement within a single field campaign, resulting in the collection of larger and more appropriate datasets, ultimately with more efficient science return.
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影响因子：
1.7
作者：
Cameron, Karen A.;Hodson, Andrew J.;Osborn, A. Mark
通讯作者：
Osborn, A. Mark
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DOI：
--
发表时间：
2004
期刊：
影响因子：
--
作者：
R. Ferla;A. Giudice;G. Maimone
通讯作者：
G. Maimone
影响因子：
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作者：
Megan J Barnett;D. Pearce;D. Cullen
通讯作者：
Megan J Barnett;D. Pearce;D. Cullen
影响因子：
2.7
作者：
Yu, Y;Lee, C;Hwang, S
通讯作者：
Hwang, S
影响因子：
4.4
作者：
Segawa, T;Miyamoto, K;Kohshima, S
通讯作者：
Kohshima, S