Two Spx regulators modulate stress tolerance and virulence in Streptococcus suis serotype 2.
Two Spx regulators modulate stress tolerance and virulence in Streptococcus suis serotype 2.
复制标题
两种 Spx 调节剂调节猪链球菌血清型 2 的应激耐受性和毒力
DOI：
10.1371/journal.pone.0108197
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Bei W
中科院分区：
文献类型：
--
作者：
Zheng C;Xu J;Li J;Hu L;Xia J;Fan J;Guo W;Chen H;Bei W
Streptococcus suis serotype 2 is an important zoonotic pathogen causing severe infections in pigs and humans. The pathogenesis of S. suis 2 infections, however, is still poorly understood. Spx proteins are a group of global regulators involved in stress tolerance and virulence. In this study, we characterized two orthologs of the Spx regulator, SpxA1 and SpxA2 in S. suis 2. Two mutant strains (ΔspxA1 and ΔspxA2) lacking the spx genes were constructed. The ΔspxA1 and ΔspxA2 mutants displayed different phenotypes. ΔspxA1 exhibited impaired growth in the presence of hydrogen peroxide, while ΔspxA2 exhibited impaired growth in the presence of SDS and NaCl. Both mutants were defective in medium lacking newborn bovine serum. Using a murine infection model, we demonstrated that the abilities of the mutant strains to colonize the tissues were significantly reduced compared to that of the wild-type strain. The mutant strains also showed a decreased level of survival in pig blood. Microarray analysis revealed a global regulatory role for SpxA1 and SpxA2. Furthermore, we demonstrated for the first time that Spx is involved in triggering the host inflammatory response. Collectively, our data suggest that SpxA1 and SpxA2 are global regulators that are implicated in stress tolerance and virulence in S. suis 2.
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影响因子：
4.9
作者：
Jousselin, Ambre;Kelley, William L.;Renzoni, Adriana
通讯作者：
Renzoni, Adriana
影响因子：
3.7
作者：
Chen C;Tang J;Dong W;Wang C;Feng Y;Wang J;Zheng F;Pan X;Liu D;Li M;Song Y;Zhu X;Sun H;Feng T;Guo Z;Ju A;Ge J;Dong Y;Sun W;Jiang Y;Wang J;Yan J;Yang H;Wang X;Gao GF;Yang R;Wang J;Yu J
通讯作者：
Yu J
影响因子：
3.1
作者：
Charland, N;Nizet, V;Gottschalk, M
通讯作者：
Gottschalk, M
影响因子：
3.2
作者：
Feng, Youjun;Li, Ming;Gao, George F.
通讯作者：
Gao, George F.
影响因子：
3.7
作者：
Chen L;Ge X;Wang X;Patel JR;Xu P
通讯作者：
Xu P