A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates.
A glimpse of streptococcal toxic shock syndrome from comparative genomics of S. suis 2 Chinese isolates.
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DOI：
10.1371/journal.pone.0000315
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发表时间：
2007-03-21
期刊：
影响因子：
3.7
通讯作者：
Yu J
中科院分区：
文献类型：
--
作者：
Chen C;Tang J;Dong W;Wang C;Feng Y;Wang J;Zheng F;Pan X;Liu D;Li M;Song Y;Zhu X;Sun H;Feng T;Guo Z;Ju A;Ge J;Dong Y;Sun W;Jiang Y;Wang J;Yan J;Yang H;Wang X;Gao GF;Yang R;Wang J;Yu J
猪链球菌2型（Streptococcus suis serotype 2，SS2）是一种重要的人畜共患病原体，在全球范围内引起了200多例严重的人类感染，主要表现为脑膜炎、败血症、关节炎等。然而，STSS的分子机制知之甚少。为阐明SS2引起STSS的遗传决定因素，对我国3株SS2进行了全基因组测序。比较基因组学伴随着几行实验，包括实验动物感染，PCR检测，和表达分析，被用来进一步剖析候选致病岛（派）。在这里，我们首次展示了对高度侵袭性SS2的中国分离株的新分子见解，该分离株在中国引起了两次大规模的人类STSS爆发。在基因组水平上研究了一个长度约为89kb的候选派，命名为89K，它是中国SS2强毒株的特异性PAI。它具有PAI的普遍特性，例如不同的GC含量，与其在STSS和高毒力中的关键作用一致。据我们所知，这是S.全世界的猪。因此，我们的发现揭示了由SS2在基因组水平上引发的STSS，有助于进一步了解其发病机制，并指出了一些有效的策略，以打击高致病性SS2感染的发展方向。
Streptococcus suis serotype 2 (SS2) is an important zoonotic pathogen, causing more than 200 cases of severe human infection worldwide, with the hallmarks of meningitis, septicemia, arthritis, etc. Very recently, SS2 has been recognized as an etiological agent for streptococcal toxic shock syndrome (STSS), which was originally associated with Streptococcus pyogenes (GAS) in Streptococci. However, the molecular mechanisms underlying STSS are poorly understood. To elucidate the genetic determinants of STSS caused by SS2, whole genome sequencing of 3 different Chinese SS2 strains was undertaken. Comparative genomics accompanied by several lines of experiments, including experimental animal infection, PCR assay, and expression analysis, were utilized to further dissect a candidate pathogenicity island (PAI). Here we show, for the first time, a novel molecular insight into Chinese isolates of highly invasive SS2, which caused two large-scale human STSS outbreaks in China. A candidate PAI of ∼89 kb in length, which is designated 89K and specific for Chinese SS2 virulent isolates, was investigated at the genomic level. It shares the universal properties of PAIs such as distinct GC content, consistent with its pivotal role in STSS and high virulence. To our knowledge, this is the first PAI candidate from S. suis worldwide. Our finding thus sheds light on STSS triggered by SS2 at the genomic level, facilitates further understanding of its pathogenesis and points to directions of development on some effective strategies to combat highly pathogenic SS2 infections.
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