Inactivation of the htpsA gene affects capsule development and pathogenicity of Streptococcus suis.
Inactivation of the htpsA gene affects capsule development and pathogenicity of Streptococcus suis.
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htpsA 基因失活影响猪链球菌荚膜发育和致病性
DOI：
10.1080/21505594.2020.1792080
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发表时间：
2020-12
期刊：
影响因子：
5.2
通讯作者：
Pan X
中科院分区：
文献类型：
--
作者：
Ni H;Li M;Wang Q;Wang J;Liu X;Zheng F;Hu D;Yu X;Han Y;Zhang Q;Zhou T;Wang Y;Wang C;Gao J;Shao ZQ;Pan X
猪链球菌血清2型（S. suis 2）是一种重要的猪病原体，也是一种新兴的人畜共患病原体。HtpsA已被报道为细菌的免疫原性细胞表面蛋白。在本研究中，我们构建了htpsA的等基因突变株ΔhtpsA，以研究其在猪链球菌2的发育和毒力中的作用。结果表明，随着唾液酸浓度的降低，突变菌株失去了其典型的包封结构。此外，在ΔhtpsA中，全血存活率、RAW264.7小鼠巨噬细胞的抗吞噬能力以及对HEp-2细胞的粘附能力均受到显著影响。此外，在小鼠感染模型中，htpsA的缺失显著降低了猪链球菌2的毒力。RNA-seq分析显示，ΔhtpsA与野生型菌株之间存在126个基因的差异表达，其中28个基因上调，98个基因下调。在下调的基因中，许多涉及碳水化合物代谢和毒力相关因子的合成。综上所述，htpsA在高毒力猪链球菌2 05ZYH33株的形态发育和发病机制中发挥了重要作用。
Streptococcus suis serotype 2 (S. suis 2) is an important swine pathogen and also an emerging zoonotic agent. HtpsA has been reported as an immunogenic cell surface protein on the bacterium. In the present study, we constructed an isogenic mutant strain of htpsA, namely ΔhtpsA, to study its role in the development and virulence of S. suis 2. Our results showed that the mutant strain lost its typical encapsulated structure with decreased concentrations of sialic acid. Furthermore, the survival rate in whole blood, the anti-phagocytosis by RAW264.7 murine macrophage, and the adherence ability to HEp-2 cells were all significantly affected in the ΔhtpsA. In addition, the deletion of htpsA sharply attenuated the virulence of S. suis 2 in an infection model of mouse. RNA-seq analysis revealed that 126 genes were differentially expressed between the ΔhtpsA and the wild-type strains, including 28 upregulated and 98 downregulated genes. Among the downregulated genes, many were involved in carbohydrate metabolism and synthesis of virulence-associated factors. Taken together, htpsA was demonstrated to play a role in the morphological development and pathogenesis of the highly virulent S. suis 2 05ZYH33 strain.
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影响因子：
3.3
作者：
Le Hong Thuy Tien;Nishibori, Tomoyuki;Osawa, Ro
通讯作者：
Osawa, Ro
影响因子：
3.8
作者：
Allen, AG;Lindsay, H;Maskell, DJ
通讯作者：
Maskell, DJ
利用RNA-Seq技术研究齐拉黑羊和和田羊卵巢中的差异基因表达。
影响因子：
3.7
作者：
Chen HY;Shen H;Jia B;Zhang YS;Wang XH;Zeng XC
通讯作者：
Zeng XC
影响因子：
3.7
作者：
Chen C;Tang J;Dong W;Wang C;Feng Y;Wang J;Zheng F;Pan X;Liu D;Li M;Song Y;Zhu X;Sun H;Feng T;Guo Z;Ju A;Ge J;Dong Y;Sun W;Jiang Y;Wang J;Yan J;Yang H;Wang X;Gao GF;Yang R;Wang J;Yu J
通讯作者：
Yu J
影响因子：
64.8
作者：
Hollenstein, Kaspar;Frei, Dominik C.;Locher, Kaspar P.
通讯作者：
Locher, Kaspar P.