Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium.
Potential localization of putative stem/progenitor cells in human bulbar conjunctival epithelium.
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DOI：
10.1002/jcp.22215
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发表时间：
2010-10
影响因子：
5.6
通讯作者：
Li DQ
中科院分区：
文献类型：
--
作者：
Qi H;Zheng X;Yuan X;Pflugfelder SC;Li DQ
虽然结膜穹窿似乎含有最大比例的干细胞，但结膜上皮干细胞袋也可能存在于整个结膜上皮中。本研究旨在通过评估6种角蛋白和13种先前提出的干细胞相关或分化标记物来研究干细胞/祖细胞在人球结膜上皮中的潜在定位。我们发现角膜特异性细胞角蛋白（CK） 3在球结膜上皮细胞中不表达。相反，CK4和CK7在球结膜上皮的浅表细胞中表达。CK14和CK15局限于基底细胞层。CK19在球结膜上皮各层均有强烈表达。人球结膜上皮基底细胞中分子标记的表达模式与角膜上皮相似。基底结膜上皮细胞强烈表达干细胞相关标志物，包括ABCG2、p63、神经生长因子（NGF）及其受体酪氨酸激酶受体A （TrkA）和神经营养因子低亲和受体p75NTR、胶质细胞源性神经营养因子（GDNF）及其受体GDNF家族受体α 1 （GFRα−1）、整合素β1、α-烯醇酶和表皮生长因子受体（EGFR）。分化相关标志物巢蛋白（nestin）、e -钙粘蛋白（E-cadherin）和天青蛋白（involucrin）均未表达。这些结果表明，球结膜上皮基底细胞具有与角膜上皮相似的干细胞相关标记物的表达模式，但具有独特的分化相关细胞角蛋白表达模式。
Although the conjunctival fornix appears to contain the greatest proportion of stem cells, it is likely that pockets of conjunctival epithelial stem cells may also exist throughout the conjunctival epithelium. This study was to investigate the potential localization of putative stem/progenitor cells in the human bulbar conjunctival epithelium by evaluating 6 keratins and 13 molecules that have been previously proposed stem cell associated or differentiation markers. We found that cornea specific cytokeratin (CK) 3 was not expressed by the bulbar conjunctival epithelial cells. In contrast, CK4 and CK7 were expressed by the superficial cells of bulbar conjunctival epithelium. CK14 and CK15 were confined to the basal cell layer. CK19 was strongly expressed by all layers of the bulbar conjunctival epithelium. The expression patterns of molecular markers in the basal cells of human bulbar conjunctival epithelium were found to be similar to the corneal epithelium. Basal conjunctival epithelial cells strongly expressed stem cell associated markers, including ABCG2, p63, nerve growth factor (NGF) with its receptors tyrosine kinase receptor A (TrkA) and neurotrophin low-affinity receptor p75NTR, glial cell-derived neurotrophic factor (GDNF) with its receptor GDNF family receptor alpha 1 (GFRα−1), integrin β1, α-enolase, and epidermal growth factor receptor (EGFR). The differentiation associated markers nestin, E-cadherin and involucrin were not expressed by these cells. These findings indicate that the basal cells of bulbar conjunctival epithelium shares a similar expression pattern of stem cell associated markers to the corneal epithelium, but has a unique pattern of differentiation associated cytokeratin expression.
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影响因子：
--
作者：
Liu, Shaohui;Li, Jing;Beuerman, Roger W.
通讯作者：
Beuerman, Roger W.
影响因子：
3.6
作者：
Buageaw, A;Sukhwani, M;Schlatt, S
通讯作者：
Schlatt, S
影响因子：
5.2
作者：
Chen, Z;De Paiva, CS;Li, DQ
通讯作者：
Li, DQ
DOI：
10.1007/bf00266749
发表时间：
1991-01-01
期刊：
HISTOCHEMISTRY
影响因子：
--
作者：
KASPER, M
通讯作者：
KASPER, M
影响因子：
2.4
作者：
Niles LP;Armstrong KJ;Rincón Castro LM;Dao CV;Sharma R;McMillan CR;Doering LC;Kirkham DL
通讯作者：
Kirkham DL