The Transcription Factor NIN-LIKE PROTEIN7 Controls Border-Like Cell Release1[OPEN]
The Transcription Factor NIN-LIKE PROTEIN7 Controls Border-Like Cell Release1[OPEN]
复制标题
转录因子 NIN-LIKE PROTEIN7 控制边界样细胞释放1[打开]
作者：
Rucha A Karve;Frank Suárez;A. Iyer
在拟南芥中，一种转录因子通过抑制细胞壁松动酶的表达来调节从根冠释放边界样细胞。根帽覆盖在根的顶端，起到保护根免受环境压力的作用。在拟南芥（Arabidopsis thaliana）中，位于根冠最后一层的细胞被称为边缘细胞或边缘样细胞（BLCs）。这些细胞从根冠的其余部分分离出来，从根冠的边缘释放出来，成为一层活细胞。BLC释放受发育调节，但其机制在很大程度上是未知的。在这里，我们发现转录因子n - like蛋白7 （NLP7）是拟南芥中blc的适当释放所必需的。NLP7的突变导致blc作为单个细胞而不是整个细胞层释放。NLP7在blc中高度表达，并在暴露于低pH时被激活，这种情况导致blc作为单个细胞释放。NLP7的突变导致纤维素和果胶水平下降。细胞壁松动酶，如CELLULASE5 （CEL5）和果胶裂解酶样基因，以及根冠调节因子SOMBRERO和bearskin /2，在nlp7-1幼苗中被激活。双突变分析显示nlp7-1表型取决于CEL5的表达水平。NLP7的突变导致对根感染真菌病原体的易感性增加。总之，这些数据表明NLP7通过细胞壁松动酶CEL5来控制blc的释放。
A transcription factor regulates the release of border-like cells from the root cap by repressing the expression of cell wall-loosening enzymes in Arabidopsis. The root cap covers the tip of the root and functions to protect the root from environmental stress. Cells in the last layer of the root cap are known as border cells, or border-like cells (BLCs) in Arabidopsis (Arabidopsis thaliana). These cells separate from the rest of the root cap and are released from its edge as a layer of living cells. BLC release is developmentally regulated, but the mechanism is largely unknown. Here, we show that the transcription factor NIN-LIKE PROTEIN7 (NLP7) is required for the proper release of BLCs in Arabidopsis. Mutations in NLP7 lead to BLCs that are released as single cells instead of an entire layer. NLP7 is highly expressed in BLCs and is activated by exposure to low pH, a condition that causes BLCs to be released as single cells. Mutations in NLP7 lead to decreased levels of cellulose and pectin. Cell wall-loosening enzymes such as CELLULASE5 (CEL5) and a pectin lyase-like gene, as well as the root cap regulators SOMBRERO and BEARSKIN1/2, are activated in nlp7-1 seedlings. Double mutant analysis revealed that the nlp7-1 phenotype depends on the expression level of CEL5. Mutations in NLP7 lead to an increase in susceptibility to a root-infecting fungal pathogen. Together, these data suggest that NLP7 controls the release of BLCs by acting through the cell wall-loosening enzyme CEL5.
影响因子：
11.8
作者：
Iyer-Pascuzzi, Anjali S.;Jackson, Terry;Cui, Hongchang;Petricka, Jalean J.;Busch, Wolfgang;Tsukagoshi, Hironaka;Benfey, Philip N.
通讯作者：
Benfey, Philip N.