A quantitative study of inhibitory interneurons in laminae I-III of the mouse spinal dorsal horn.
A quantitative study of inhibitory interneurons in laminae I-III of the mouse spinal dorsal horn.
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DOI：
10.1371/journal.pone.0078309
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Todd AJ
中科院分区：
文献类型：
--
作者：
Polgár E;Durrieux C;Hughes DI;Todd AJ
脊髓背角的I-III层含有许多使用GABA和/或甘氨酸作为神经递质的抑制性中间神经元。在这些细胞中可以识别出不同的神经化学群体，这些群体在抑制疼痛或瘙痒方面可能具有不同的作用。大鼠的定量研究表明，抑制性中间神经元占该区域所有神经元的25-40%。Sst 2A受体由板层I-II中约一半的抑制性中间神经元表达，并且与特定的神经化学定义的群体相关。虽然大部分关于脊髓疼痛机制的研究都是在大鼠身上进行的，但由于基因改变的品系的可用性，小鼠现在越来越多地被用作模型。然而，在小鼠中缺乏关于中间神经元排列的定量信息，并且神经元组织可能存在显著的种属差异。在这项研究中，我们表明，在大鼠中，几乎所有的神经元在板层I-III富含甘氨酸也含有GABA，这表明GABA-免疫反应性可用于识别抑制性中间神经元在这一地区。这些细胞占I-II层神经元的26%，占III层神经元的38%。在大鼠中，sst 2A受体仅由板层I-II中的抑制性中间神经元表达，并且存在于这些细胞的一半以上（54%）。针对神经激肽1受体的抗体被用来定义板层I，我们发现，虽然受体集中在这个板层，它是由比在大鼠少得多的细胞表达。通过估计小鼠L4节段中每个椎板中的神经元总数，我们发现每个椎板中的神经元数量约为大鼠相应节段中神经元数量的一半。
Laminae I-III of the spinal dorsal horn contain many inhibitory interneurons that use GABA and/or glycine as a neurotransmitter. Distinct neurochemical populations can be recognised among these cells, and these populations are likely to have differing roles in inhibiting pain or itch. Quantitative studies in rat have shown that inhibitory interneurons account for 25-40% of all neurons in this region. The sst2A receptor is expressed by around half the inhibitory interneurons in laminae I-II, and is associated with particular neurochemically-defined populations. Although much of the work on spinal pain mechanisms has been performed on rat, the mouse is now increasingly used as a model, due to the availability of genetically altered lines. However, quantitative information on the arrangement of interneurons is lacking in the mouse, and it is possible that there are significant species differences in neuronal organisation. In this study, we show that as in the rat, nearly all neurons in laminae I-III that are enriched with glycine also contain GABA, which suggests that GABA-immunoreactivity can be used to identify inhibitory interneurons in this region. These cells account for 26% of the neurons in laminae I-II and 38% of those in lamina III. As in the rat, the sst2A receptor is only expressed by inhibitory interneurons in laminae I-II, and is present on just over half (54%) of these cells. Antibody against the neurokinin 1 receptor was used to define lamina I, and we found that although the receptor was concentrated in this lamina, it was expressed by many fewer cells than in the rat. By estimating the total numbers of neurons in each of these laminae in the L4 segment of the mouse, we show that there are around half as many neurons in each lamina as are present in the corresponding segment of the rat.
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