Genetic analysis of cryptochrome in insect magnetosensitivity.
Genetic analysis of cryptochrome in insect magnetosensitivity.
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DOI：
10.3389/fphys.2022.928416
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发表时间：
2022
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
作者：
地球磁场对许多高等动物的壮观迁徙和导航能力起着重要作用，尤其是鸟类。然而，与模型果蝇不同，这些生物不能进行遗传分析，果蝇在实验室条件下可以对磁场做出反应。因此，我们回顾了昆虫磁敏性的领域，重点是隐花色素(CRY)的作用，这是最先在拟南芥和果蝇中发现的，是昼夜光携带途径的关键分子成分。物理化学研究表明，结合CREY的黄素腺嘌呤二核苷酸(FAD)的光激活会产生FADO−TrPO+自由基对，因为电子沿着特定的Trp残基链跳跃，并且这些自由基的量子自旋化学对磁场很敏感。利用基因编辑、替换/抢救和基因敲除的方法对几种昆虫的哭声进行了操作。这些不同的突变对磁敏感性的影响揭示了一些令人惊讶的事情，根据体内和体外研究的最新进展进行了讨论。
The earth’s magnetic field plays an important role in the spectacular migrations and navigational abilities of many higher animals, particularly birds. However, these organisms are not amenable to genetic analysis, unlike the model fruitfly, Drosophila melanogaster, which can respond to magnetic fields under laboratory conditions. We therefore review the field of insect magnetosensitivity focusing on the role of the Cryptochromes (CRYs) that were first identified in Arabidopsis and Drosophila as key molecular components of circadian photo-entrainment pathways. Physico-chemical studies suggest that photo-activation of flavin adenine dinucleotide (FAD) bound to CRY generates a FADo− Trpo+ radical pair as electrons skip along a chain of specific Trp residues and that the quantum spin chemistry of these radicals is sensitive to magnetic fields. The manipulation of CRY in several insect species has been performed using gene editing, replacement/rescue and knockdown methods. The effects of these various mutations on magnetosensitivity have revealed a number of surprises that are discussed in the light of recent developments from both in vivo and in vitro studies.
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电磁场通过哭泣的依赖性途径破坏了果蝇中的负岩石。
影响因子：
16.6
作者：
Fedele, Giorgio;Green, Edward W.;Rosato, Ezio;Kyriacou, Charalambos P.
通讯作者：
Kyriacou, Charalambos P.
影响因子：
5.7
作者：
Antill, Lewis M.;Woodward, Jonathan R.
通讯作者：
Woodward, Jonathan R.
影响因子：
4.5
作者：
Fedele G;Edwards MD;Bhutani S;Hares JM;Murbach M;Green EW;Dissel S;Hastings MH;Rosato E;Kyriacou CP
通讯作者：
Kyriacou CP
影响因子：
4.8
作者：
Berndt, Alex;Kottke, Tilman;Wolf, Eva
通讯作者：
Wolf, Eva
影响因子：
27.5
作者：
Hoang, Nathalie;Bouly, Jean-Pierre;Ahmad, Margaret
通讯作者：
Ahmad, Margaret