QTL mapping and GWAS reveal candidate genes controlling capsaicinoid content in Capsicum.
QTL mapping and GWAS reveal candidate genes controlling capsaicinoid content in Capsicum.
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DOI：
10.1111/pbi.12894
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发表时间：
2018-02-06
影响因子：
13.8
通讯作者：
Kang BC
中科院分区：
文献类型：
--
作者：
Han K;Lee HY;Ro NY;Hur OS;Lee JH;Kwon JK;Kang BC
辣椒素是仅在辣椒（辣椒属）中产生的独特化合物。一些研究使用经典的数量性状基因座（QTL）定位和全基因组关联研究（GWAS）已经确定了控制辣椒中辣椒素含量的QTL;然而，由于使用的每种方法的局限性，既没有确定每个群体共有的QTL，也没有确定潜在的候选基因。在这里，我们进行了QTL定位和GWAS辣椒类辣椒素含量使用两个重组自交系（RIL）人口和一个GWAS人口。使用全基因组重测序和测序基因分型（GBS）构建高密度单核苷酸多态性（SNP）图谱。5个QTL区域上的第1，2，3，4和10号染色体上的两个RIL群体在多个地点和多年的共同确定。此外，来自两个GBS文库的总共109 610个SNP被用于分析由208个C. annuum-clade加入组成的GWAS群体。从GWAS中共鉴定了69个QTL区域，其中10个与从两个双亲群体中鉴定的QTL共定位。在这些区域内，我们能够确定已知参与辣椒素生物合成的五个候选基因。我们的研究结果表明，QTL定位和GBS‐GWAS代表了一种强大的组合方法，用于识别控制复杂性状的基因座。
Capsaicinoids are unique compounds produced only in peppers (Capsicum spp.). Several studies using classical quantitative trait loci (QTLs) mapping and genomewide association studies (GWAS) have identified QTLs controlling capsaicinoid content in peppers; however, neither the QTLs common to each population nor the candidate genes underlying them have been identified due to the limitations of each approach used. Here, we performed QTL mapping and GWAS for capsaicinoid content in peppers using two recombinant inbred line (RIL) populations and one GWAS population. Whole‐genome resequencing and genotyping by sequencing (GBS) were used to construct high‐density single nucleotide polymorphism (SNP) maps. Five QTL regions on chromosomes 1, 2, 3, 4 and 10 were commonly identified in both RIL populations over multiple locations and years. Furthermore, a total of 109 610 SNPs derived from two GBS libraries were used to analyse the GWAS population consisting of 208 C. annuum‐clade accessions. A total of 69 QTL regions were identified from the GWAS, 10 of which were co‐located with the QTLs identified from the two biparental populations. Within these regions, we were able to identify five candidate genes known to be involved in capsaicinoid biosynthesis. Our results demonstrate that QTL mapping and GBS‐GWAS represent a powerful combined approach for the identification of loci controlling complex traits.
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影响因子：
16.6
作者：
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通讯作者：
McCouch S
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