Microbiome Responses to an Uncontrolled Short-Term Diet Intervention in the Frame of the Citizen Science Project.
Microbiome Responses to an Uncontrolled Short-Term Diet Intervention in the Frame of the Citizen Science Project.
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DOI：
10.3390/nu10050576
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发表时间：
2018-05-08
期刊：
影响因子：
5.9
通讯作者：
Alexeev DG
中科院分区：
文献类型：
--
作者：
Klimenko NS;Tyakht AV;Popenko AS;Vasiliev AS;Altukhov IA;Ischenko DS;Shashkova TI;Efimova DA;Nikogosov DA;Osipenko DA;Musienko SV;Selezneva KS;Baranova A;Kurilshikov AM;Toshchakov SM;Korzhenkov AA;Samarov NI;Shevchenko MA;Tepliuk AV;Alexeev DG
积极监测自身健康的个人对个性化营养越来越感兴趣。饮食干预的持续时间与对肠道微生物群的影响之间的关系尚未阐明。在这里，我们研究了短期饮食变化、长期饮食习惯和生活方式与肠道微生物群的关系。在自我报告的为期两周的个性化饮食干预前后收集了 248 名公民科学志愿者的粪便样本，然后使用 16S rRNA 测序进行分析。研究发现长期饮食习惯与肠道群落结构之间存在显着相关性。蔬菜和水果摄入量越高，产生丁酸盐的梭状芽胞杆菌水平越高，群落丰富度越高。对两周干预前后的宏基因组进行配对比较表明，即使是短暂的、不受控制的干预也会对群落结构产生深刻的变化：导致拟杆菌科、卟啉单胞菌科和理肯氏菌科的水平下降，α-多样性减少，同时产甲烷短杆菌、双歧杆菌、梭状芽胞杆菌和产生丁酸的毛螺旋菌科增加，以及永久型（a基于引导的肠型变异）与更高的饮食多样性相关。微生物群对干预的反应取决于初始微生物群状态。这些发现为个性化饮食的发展铺平了道路。
Personalized nutrition is of increasing interest to individuals actively monitoring their health. The relations between the duration of diet intervention and the effects on gut microbiota have yet to be elucidated. Here we examined the associations of short-term dietary changes, long-term dietary habits and lifestyle with gut microbiota. Stool samples from 248 citizen-science volunteers were collected before and after a self-reported 2-week personalized diet intervention, then analyzed using 16S rRNA sequencing. Considerable correlations between long-term dietary habits and gut community structure were detected. A higher intake of vegetables and fruits was associated with increased levels of butyrate-producing Clostridiales and higher community richness. A paired comparison of the metagenomes before and after the 2-week intervention showed that even a brief, uncontrolled intervention produced profound changes in community structure: resulting in decreased levels of Bacteroidaceae, Porphyromonadaceae and Rikenellaceae families and decreased alpha-diversity coupled with an increase of Methanobrevibacter, Bifidobacterium, Clostridium and butyrate-producing Lachnospiraceae- as well as the prevalence of a permatype (a bootstrapping-based variation of enterotype) associated with a higher diversity of diet. The response of microbiota to the intervention was dependent on the initial microbiota state. These findings pave the way for the development of an individualized diet.
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使用通用的unifrac距离将微生物组组成与环境协变量相关联。
DOI：
10.1093/bioinformatics/bts342
发表时间：
2012-08-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Chen J;Bittinger K;Charlson ES;Hoffmann C;Lewis J;Wu GD;Collman RG;Bushman FD;Li H
通讯作者：
Li H
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
2.9
作者：
Egshatyan L;Kashtanova D;Popenko A;Tkacheva O;Tyakht A;Alexeev D;Karamnova N;Kostryukova E;Babenko V;Vakhitova M;Boytsov S
通讯作者：
Boytsov S
影响因子：
15.5
作者：
Fernandes AD;Reid JN;Macklaim JM;McMurrough TA;Edgell DR;Gloor GB
通讯作者：
Gloor GB
影响因子：
15.5
作者：
Dubinkina VB;Tyakht AV;Odintsova VY;Yarygin KS;Kovarsky BA;Pavlenko AV;Ischenko DS;Popenko AS;Alexeev DG;Taraskina AY;Nasyrova RF;Krupitsky EM;Shalikiani NV;Bakulin IG;Shcherbakov PL;Skorodumova LO;Larin AK;Kostryukova ES;Abdulkhakov RA;Abdulkhakov SR;Malanin SY;Ismagilova RK;Grigoryeva TV;Ilina EN;Govorun VM
通讯作者：
Govorun VM