Do transgenesis and marker-assisted backcross breeding produce substantially equivalent plants? A comparative study of transgenic and backcross rice carrying bacterial blight resistant gene Xa21.
Do transgenesis and marker-assisted backcross breeding produce substantially equivalent plants? A comparative study of transgenic and backcross rice carrying bacterial blight resistant gene Xa21.
复制标题
转基因和标记辅助回交育种是否能产生基本相同的植物？
DOI：
10.1186/1471-2164-14-738
复制
发表时间：
2013-10-29
期刊：
影响因子：
4.4
通讯作者：
Zhai W
中科院分区：
文献类型：
--
作者：
Gao L;Cao Y;Xia Z;Jiang G;Liu G;Zhang W;Zhai W
背景
转基因植物对人类健康的潜在影响已引起全世界的广泛关注，但这一问题仍存在争议。这与通过标记辅助回交（MAB）育种产生的植物的广泛接受形成鲜明对比。
结果
聚焦于转录组变异和对信号通路的干扰，我们评估了转基因作物和MAB育种植物之间实质等同性的分子和生物学方面，这是粮食及农业组织和世界卫生组织认可的食品安全的一般原则。我们比较了转基因水稻品系（DXT）和MAB水稻品系（DXB），两者都含有提供抗白叶枯病的基因Xa 21。利用DXT、DXB及其亲本D 62 B的Next-Generation测序数据，比较了DXT和DXB的转录组变异。值得注意的是，DXT的差异表达基因（DEG）比DXB少43%。DXT和DXB中特异表达的基因具有病原和胁迫防御功能。DXT中DEG的功能类别与DXB中的DEG的功能类别相当，并且受转基因显著影响的11条途径中的7条也受到MAB育种的干扰。
结论
这些结果表明，转基因水稻和MAB育种的水稻在转录组水平上是基本等同的，为转基因水稻的进一步研究奠定了基础，了解目前研究中确定的DEG的化学和营养特性。
Background
The potential impact of genetically modified (GM) plants on human health has attracted much attention worldwide, and the issue remains controversial. This is in sharp contrast to the broad acceptance of plants produced by breeding through Marker Assisted Backcrossing (MAB).
Results
Focusing on transcriptome variation and perturbation to signaling pathways, we assessed the molecular and biological aspects of substantial equivalence, a general principle for food safety endorsed by the Food and Agricultural Organization and the World Health Organization, between a transgenic crop and a plant from MAB breeding. We compared a transgenic rice line (DXT) and a MAB rice line (DXB), both of which contain the gene Xa21 providing resistance to bacterial leaf blight. By using Next-Generation sequencing data of DXT, DXB and their parental line (D62B), we compared the transcriptome variation of DXT and DXB. Remarkably, DXT had 43% fewer differentially expressed genes (DEGs) than DXB. The genes exclusively expressed in DXT and in DXB have pathogen and stress defense functions. Functional categories of DEGs in DXT were comparable to that in DXB, and seven of the eleven pathways significantly affected by transgenesis were also perturbed by MAB breeding.
Conclusions
These results indicated that the transgenic rice and rice from MAB breeding are substantial equivalent at the transcriptome level, and paved a way for further study of transgenic rice, e.g., understanding the chemical and nutritional properties of the DEGs identified in the current study.
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DOI：
10.1073/pnas.93.15.7783
发表时间：
1996-07-23
影响因子：
11.1
作者：
Hirochika, H;Sugimoto, K;Kanda, M
通讯作者：
Kanda, M
影响因子：
4.2
作者：
Akimoto, Keiko;Katakami, Hatsue;Sano, Hiroshi
通讯作者：
Sano, Hiroshi
DOI：
10.1073/pnas.2435133100
发表时间：
2003-12-09
影响因子：
11.1
作者：
Feuillet, C;Travella, S;Keller, B
通讯作者：
Keller, B
影响因子：
2
作者：
Gao, Lifen;Xia, Zhihui;Zhai, Wenxue
通讯作者：
Zhai, Wenxue
影响因子：
11.4
作者：
Li, Yuan;Xia, Qiong;Liu, Bao
通讯作者：
Liu, Bao