The adaptive remodeling of endothelial glycocalyx in response to fluid shear stress.
The adaptive remodeling of endothelial glycocalyx in response to fluid shear stress.
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DOI：
10.1371/journal.pone.0086249
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Tarbell JM
中科院分区：
文献类型：
--
作者：
Zeng Y;Tarbell JM
内皮糖萼对于机械传导和内皮屏障完整性至关重要。我们之前证明了在剪切暴露的最初30分钟内糖萼组织的早期变化。在本研究中，我们测试的假设，长期剪切应力诱导进一步重塑的糖萼导致一个强大的层，并探讨了膜筏和肌动蛋白细胞骨架的反应。暴露于剪切应力24小时后，糖萼组分硫酸乙酰肝素，硫酸软骨素，磷脂酰肌醇蛋白聚糖-1和多配体蛋白聚糖-1，在顶端表面上增强，与接近基线水平的几乎均匀的空间分布，大大不同于30分钟的分布。硫酸乙酰肝素和磷脂酰肌醇蛋白聚糖-1仍然聚集在细胞边界附近的剪切24小时后，但小窝蛋白-1/小窝和肌动蛋白增强，并集中在整个顶端方面的细胞。我们的研究结果还表明，GM 1标记的膜筏与小窝和磷脂酰肌醇蛋白聚糖-1/硫酸乙酰肝素，并在演唱会与这些组件的变化。我们的结论是，重塑的糖萼长期剪切应力与膜筏和肌动蛋白细胞骨架的变化。这项研究揭示了一个空间和时间依赖性重组的糖萼，可能改变的机械转导机制在剪切暴露的时间过程。
The endothelial glycocalyx is vital for mechanotransduction and endothelial barrier integrity. We previously demonstrated the early changes in glycocalyx organization during the initial 30 min of shear exposure. In the present study, we tested the hypothesis that long-term shear stress induces further remodeling of the glycocalyx resulting in a robust layer, and explored the responses of membrane rafts and the actin cytoskeleton. After exposure to shear stress for 24 h, the glycocalyx components heparan sulfate, chondroitin sulfate, glypican-1 and syndecan-1, were enhanced on the apical surface, with nearly uniform spatial distributions close to baseline levels that differed greatly from the 30 min distributions. Heparan sulfate and glypican-1 still clustered near the cell boundaries after 24 h of shear, but caveolin-1/caveolae and actin were enhanced and concentrated across the apical aspects of the cell. Our findings also suggest the GM1-labelled membrane rafts were associated with caveolae and glypican-1/heparan sulfate and varied in concert with these components. We conclude that remodeling of the glycocalyx to long-term shear stress is associated with the changes in membrane rafts and the actin cytoskeleton. This study reveals a space- and time- dependent reorganization of the glycocalyx that may underlie alterations in mechanotransduction mechanisms over the time course of shear exposure.
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DOI：
10.1152/ajpheart.00592.2005
发表时间：
2006-01-01
影响因子：
4.8
作者：
Gouverneur, M;Spaan, JAE;Vink, H
通讯作者：
Vink, H
DOI：
10.1152/ajpheart.01047.2006
发表时间：
2007-03-01
影响因子：
4.8
作者：
Chien, Shu
通讯作者：
Chien, Shu
影响因子：
12.3
作者：
Filmus J;Capurro M;Rast J
通讯作者：
Rast J
从内皮丰富的来源分离的富含小窝素膜结构域的表征：对人类疾病的影响。
影响因子：
7.8
作者：
Lisanti, M P;Scherer, P E;Vidugiriene, J;Tang, Z;Hermanowski-Vosatka, A;Tu, Y H;Cook, R F;Sargiacomo, M
通讯作者：
Sargiacomo, M
影响因子：
5.5
作者：
Koo, Andrew;Dewey, C. Forbes, Jr.;Garcia-Cardena, Guillermo
通讯作者：
Garcia-Cardena, Guillermo