Neuroprotective Effects of Asparagus officinalis Stem Extract in Transgenic Mice Overexpressing Amyloid Precursor Protein.
Neuroprotective Effects of Asparagus officinalis Stem Extract in Transgenic Mice Overexpressing Amyloid Precursor Protein.
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芦笋茎提取物在过表达淀粉样蛋白前体蛋白的转基因小鼠中的神经保护作用。
DOI：
10.1155/2021/8121407
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发表时间：
2021
影响因子：
4.1
通讯作者：
Doursout MF
中科院分区：
文献类型：
--
作者：
Peng Z;Bedi S;Mann V;Sundaresan A;Homma K;Gaskey G;Kowada M;Umar S;Kulkarni AD;Eltzschig HK;Doursout MF
为了模拟阿尔茨海默氏病，在本研究中使用过表达淀粉样前体蛋白（APP）的转基因小鼠。我们假设ETAS®50（Amino Up Co.生产的芦笋茎标准化提取物）的神经保护作用，Ltd.（札幌，日本）的热休克蛋白（HSP）与通过增强应激反应蛋白抑制凋亡级联有关。APP-过表达小鼠（具有129 s6背景的APP和PS1双转基因小鼠品系），年龄6-8周龄，体重20-24克，在我们的实验室中成功繁殖。将动物分为5组。APP过表达小鼠和野生型（WT）小鼠用ETAS®50粉末（50%元素ETAS和50% destrin）以200 mg/kg和1000 mg/kg体重进行预处理。在APP过表达小鼠和WT小鼠中给予ETAS®50的溶剂生理盐水。每天灌胃给予ETAS®50和生理盐水，持续1个月。使用Morris水迷宫进行的认知评估表明，与未治疗的APP小鼠相比，ETAS®50治疗后记忆恢复。在安乐死时，取出脑，并通过用适当的抗体进行免疫荧光染色来评价HSP、淀粉样蛋白β、tau蛋白和半胱天冬酶-3。我们的数据表明，APP小鼠具有认知障碍沿着淀粉样蛋白β、tau蛋白和半胱天冬酶-3升高。ETAS®50恢复了这些转基因小鼠的认知功能，增加了HSP 70和HSP 27，并降低了淀粉样蛋白β、tau蛋白和半胱天冬酶-3的致病水平，从而产生神经保护作用。我们的研究结果证实了海马HSP 70基因表达的显着增加。
To mimic Alzheimer's disease, transgenic mice overexpressing the amyloid precursor protein (APP) were used in this study. We hypothesize that the neuroprotective effects of ETAS®50, a standardized extract of Asparagus officinalis stem produced by Amino Up Co., Ltd. (Sapporo, Japan), are linked to the inhibition of the apoptosis cascade through an enhancement of the stress-response proteins: heat shock proteins (HSPs). APP-overexpressing mice (double-transgenic APP and PS1 mouse strains with a 129s6 background), ages 6-8 weeks old, and weighing 20-24 grams were successfully bred in our laboratory. The animals were divided into 5 groups. APP-overexpressing mice and wild-type (WT) mice were pretreated with ETAS®50 powder (50% elemental ETAS and 50% destrin) at 200 mg/kg and 1000 mg/kg body weight. Saline, the vehicle for ETAS®50, was administered in APP-overexpressing mice and WT mice. ETAS®50 and saline were administered by gavage daily for 1 month. Cognitive assessments, using the Morris Water Maze, demonstrated that memory was recovered following ETAS®50 treatment as compared to nontreated APP mice. At euthanization, the brain was removed and HSPs, amyloid β, tau proteins, and caspase-3 were evaluated through immunofluorescence staining with the appropriate antibodies. Our data indicate that APP mice have cognitive impairment along with elevated amyloid β, tau proteins, and caspase-3. ETAS®50 restored cognitive function in these transgenic mice, increased both HSP70 and HSP27, and attenuated pathogenic level of amyloid β, tau proteins, and caspsase-3 leading to neuroprotection. Our results were confirmed with a significant increase in HSP70 gene expression in the hippocampus.
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DOI：
10.1016/s0169-328x(96)00138-6
发表时间：
1997-05-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
Foster, JA;Brown, IR
通讯作者：
Brown, IR
影响因子：
11.4
作者：
Jäättelä, M;Wissing, D;Egeblad, M
通讯作者：
Egeblad, M
影响因子：
3.3
作者：
Hussein AM;Adel M;El-Mesery M;Abbas KM;Ali AN;Abulseoud OA
通讯作者：
Abulseoud OA
DOI：
10.3233/jad-160612
发表时间：
2017
期刊：
Journal of Alzheimer's disease : JAD
影响因子：
--
作者：
Rajmohan R;Reddy PH
通讯作者：
Reddy PH
DOI：
10.1152/ajpregu.00513.2002
发表时间：
2003-02-01
影响因子：
2.8
作者：
Campisi, J;Leem, TH;Fleshner, M
通讯作者：
Fleshner, M