Characterization of gastric mucosal blood flow response induced by intragastric capsaicin in rats.
Characterization of gastric mucosal blood flow response induced by intragastric capsaicin in rats.
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大鼠胃内辣椒素诱导的胃粘膜血流反应的特征。
DOI：
10.1254/jjp.57.205
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
S. Okabe
中科院分区：
文献类型：
--
作者：
J. Matsumoto;K. Takeuchi;S. Okabe
在麻醉大鼠离体胃同时测定胃粘膜血流量(GMBF)、平均动脉压(MBP)、管腔pH和跨粘膜电位差(PD)，以探讨辣椒素引起的上述反应。胃固定在Lucite小室上，以1ml/min的流速注入生理盐水，用激光血流仪测量GMBF。在此条件下，pH为3.5~4.0，PD为-30~-35 mV，GMBF为8~12ml/min/100g。黏膜应用辣椒素(0.03-1 mg/ml，作用10min)可浓度依赖性地增加GMBF，但对PD、pH和MBP无明显影响。全身注射辣椒素(总剂量：100 mg/kg)后，辣椒素引起的GMBF反应可被化学去传入所消除，而预先注射吲哚美辛(5 mg/kg，S.C.)可显著减弱辣椒素引起的GMBF反应。或Ru红(300微克/公斤，静脉注射)，但不受斯潘替丁(100微克/公斤，静脉注射)、阿托品(300微克/公斤，静脉注射)的影响。或色甘酸二钠(30 mg/kg，ip)。此外，当粘膜反复接触辣椒素时，这种反应在高浓度(6 mg/ml)时表现出明显的快速反应。这些结果提示，胃内辣椒素通过辣椒素敏感的感觉神经元选择性地增加GMBF，这种作用可能与内源性前列腺素有关。
Gastric mucosal blood flow (GMBF) was measured in the ex vivo stomachs of anesthetized rats simultaneously with mean arterial blood pressure (MBP), luminal pH and transmucosal potential difference (PD) in an attempt to characterize these responses induced by capsaicin. The stomach was mounted on a Lucite chamber, perfused with saline at the flow rate of 1 ml/min, and GMBF was measured by Laser flowmetry. Under these conditions, the pH, PD and GMBF were 3.5 to 4.0, -30 to -35 mV and 8-12 ml/min/100 g, respectively. Mucosal application of capsaicin (0.03 - 1 mg/ml for 10 min) increased GMBF in a concentration-dependent manner, without any change in PD, pH and MBP. The increased GMBF response caused by capsaicin was abolished by chemical deafferentation following systemic capsaicin injections (total dose: 100 mg/kg), significantly attenuated by pretreatment with indomethacin (5 mg/kg, s.c.) or ruthenium red (300 micrograms/kg, i.v.), but was not affected by spantide (100 micrograms/kg, i.v.), atropine (300 micrograms/kg, i.p.) or disodium cromoglycate (30 mg/kg, i.p.). In addition, when the mucosa was exposed to capsaicin repeatedly, this response showed a marked tachyphylaxis at a high concentration (6 mg/ml). These results suggest that intragastric capsaicin increased GMBF selectively through capsaicin-sensitive sensory neurons, and this action may involve endogenous prostaglandins.
影响因子：
21.1
作者：
S. H. Buck;T. Burks
通讯作者：
S. H. Buck;T. Burks
剖腹手术诱导的胃对乙醇损伤的保护是由辣椒素敏感的感觉神经元介导的。
影响因子：
29.4
作者：
Yonei,Y;Holzer,P;Guth,PH
通讯作者：
Guth,PH