Diffuse type of senile plaques in the brains of Alzheimer-type dementia
Diffuse type of senile plaques in the brains of Alzheimer-type dementia
复制标题
阿尔茨海默型痴呆患者大脑中弥漫性老年斑
作者：
Haruyasu Yamaguchi;Shunsaku Hirai;M. Morimatsu;Mikio Shoji;Y. Harigaya
我们研究了6例阿尔茨海默型痴呆（ATD）尸检者脑内弥漫型老年斑（SP）的性质。用四种不同方法染色的连续切片评价内嗅皮质中SP的密度。与β蛋白免疫染色（100%作为参考）相比，改良Bielschowsky染色（103%）和高碘酸-六亚甲基四胺银（PAM）染色（109%）标记了相似数量的SP，而Bodian染色仅标记了其中的一小部分（42%）。绝大多数Bodian阴性斑块为弥漫性斑块，经甲酸预处理的β蛋白免疫染色、改良Bielschowsky染色和PAM染色后，可见不清晰的细纤维状物质区域。它们未被刚果红或高碘酸-希夫染色剂染色。Bodian和β蛋白双重染色显示弥漫性斑块无肿胀的神经突。当切片用甲酸预处理时，通过改良的Bielschowsky和PAM染色显示的弥漫性斑块的嗜银性变得不可检测。这种处理使弥漫性斑块对β蛋白抗血清呈免疫反应性，表明弥漫性斑块主要由淀粉样蛋白组成，而不是神经炎成分。弥漫性斑块分布在各个皮质区和杏仁核中，并且在ATD脑中包括相当数量的SP。
SummaryWe studied the nature of diffuse type of senile plaques (SP) in the brains of six autopsied subjects with Alzheimer-type dementia (ATD). The densities of SP in the entorhinal cortex were evaluated using serial sections stained by four different methods. Compared with β protein immunostaining (100% as a reference), the modified Bielschowsky stain (103%) and the periodic acid-methenamine silver (PAM) stain (109%) labeled similar numbers of SP, whereas the Bodian stain labeled only a minor proportion (42%) of these. The vast majority of Bodian-negative plaques were diffuse plaques, which were seen as ill-defined areas of fine fibrillar material after β protein immunostain with formic acid pretreatment, modified Bielschowsky stain, and PAM stain. They were not stained by Congo red or periodic acid-Schiff stains. Double staining using Bodian and β protein methods demonstrated that diffuse plaques were free of swollen neurites. Argyrophilia of the diffuse plaques shown by the modified Bielschowsky and PAM stains, became undetectable when sections were pretreated with formic acid. Such treatment made the diffuse plaques immunoreactive to β protein antiserum, suggesting that diffuse plaques consisted mainly of amyloid, but not neuritic components. The diffuse plaques were distributed in various cortical areas and in the amygdala, and comprised a considerable population of the SP in the ATD brains.
影响因子：
56.9
作者：
SELKOE, DJ;BELL, DS;CORK, LC
通讯作者：
CORK, LC