Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
Rapid granulation tissue regeneration by intracellular ATP delivery--a comparison with Regranex.
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DOI：
10.1371/journal.pone.0091787
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Chien S
中科院分区：
文献类型：
--
作者：
Howard JD;Sarojini H;Wan R;Chien S
本研究测试了一种新的细胞内ATP递送技术用于组织再生，并将其与Regranex的效果进行了比较。27只成年新西兰大白兔均行微创手术使一只耳朵缺血。然后创造了8个伤口：4个在缺血耳上，4个在正常耳上。双耳一侧创面用Mg-ATP包封脂质囊泡（atp -囊泡）处理，另一侧创面用Regranex处理。使用atp囊泡时，伤口愈合时间较短。最引人注目的发现是，当使用atp囊泡时，新组织在不到1天的时间内开始出现。在几天内，生长继续并覆盖了伤口区域，没有形成临时基质。regranex处理过的伤口没有这种生长模式。在atp -囊泡处理的伤口中，组织学研究显示巨噬细胞积聚异常丰富，增殖细胞核抗原（PCNA）活跃，BrdU染色阳性，表明巨噬细胞原位增殖。人巨噬细胞培养提示直接产生胶原蛋白。这些结果支持一种全新的愈合过程，它似乎将传统的止血、炎症和增殖阶段合并为一个阶段，从而消除了愈合过程中通常出现的滞后时间。
This study tests a new intracellular ATP delivery technique for tissue regeneration and compares its efficacy with that of Regranex. Twenty-seven adult New Zealand white rabbits each underwent minimally invasive surgery to render one ear ischemic. Eight wounds were then created: four on the ischemic and four on the normal ear. Two wounds on one side of each ear were treated with Mg-ATP encapsulated lipid vesicles (ATP-vesicles) while the two wounds on the other side were treated with Regranex. Wound healing time was shorter when ATP-vesicles were used. The most striking finding was that new tissue growth started to appear in less than 1 day when ATP-vesicles were used. The growth continued and covered the wound area within a few days, without the formation of a provisional matrix. Regranex-treated wounds did not have this growth pattern. In wounds treated by ATP-vesicles, histologic studies revealed extremely rich macrophage accumulation, along with active proliferating cell nuclear antigen (PCNA) and positive BrdU staining, indicating in situ macrophage proliferation. Human macrophage culture suggested direct collagen production. These results support an entirely new healing process, which seems to have combined the conventional hemostasis, inflammation, and proliferation phases into a single one, thereby eliminating the lag time usually seen during healing process.
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影响因子：
11.8
作者：
Berendt, A. R.
通讯作者：
Berendt, A. R.
影响因子：
4.6
作者：
Cheng, Biao;Liu, Hong-Wei;Sheng, Zhi-Yong
通讯作者：
Sheng, Zhi-Yong
影响因子：
8
作者：
Game, F. L.;Hinchliffe, R. J.;Jeffcoate, W. J.
通讯作者：
Jeffcoate, W. J.
影响因子：
2.9
作者：
Kloeters, Oliver;Tandara, Andrea;Mustoe, Thomas A.
通讯作者：
Mustoe, Thomas A.
DOI：
10.1007/978-1-60327-360-2_26
发表时间：
2010-01-01
期刊：
LIPOSOMES: METHODS AND PROTOCOLS, VOL 1
影响因子：
--
作者：
Chien, Sufan
通讯作者：
Chien, Sufan