Endogenous rRNA Sequence Variation Can Regulate Stress Response Gene Expression and Phenotype.
Endogenous rRNA Sequence Variation Can Regulate Stress Response Gene Expression and Phenotype.
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DOI：
10.1016/j.celrep.2018.08.093
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发表时间：
2018-10-02
期刊：
影响因子：
8.8
通讯作者：
Blanchard SC
中科院分区：
文献类型：
--
作者：
Kurylo CM;Parks MM;Juette MF;Zinshteyn B;Altman RB;Thibado JK;Vincent CT;Blanchard SC
流行的教条认为，核糖体在组成和功能上是一致的。在这里，我们证明了营养限制诱导的胁迫在大肠杆菌中改变了rDNA操纵子的相对表达，从而改变了活跃翻译核糖体池中的rRNA组成。最上调的操纵子编码独特的16S rRNA，rrsH，其特点是小核糖体亚基内保守的序列变异。携带rrsH的核糖体影响功能上一致的基因集的表达，并改变rpos sigma因子的水平，rpos sigma因子是一般应激反应的主要调节因子。这些影响与抗生素敏感性、生物膜形成和细胞运动性的表型变化有关，并受到应激反应蛋白RELA和RERE以及代谢酶和毒力相关蛋白ADHE的调节。这些发现证实，内源性编码的、自然发生的rRNA序列变异可以调节核糖体功能、基因表达调控的中心方面和细胞生理。大多数生物编码多个不同的rRNA基因拷贝，使得核糖体池的组成具有内在的异质性。在这里，Kurylo等人。结果表明，在16S rRNA中，营养限制上调了含有保守序列变异的核糖体的表达，从而调节了基因的表达和表型。
Prevailing dogma holds that ribosomes are uniform in composition and function. Here, we show that nutrient limitation-induced stress in E. coli changes the relative expression of rDNA operons to alter the rRNA composition within the actively translating ribosome pool. The most upregulated operon encodes the unique 16S rRNA, rrsH, distinguished by conserved sequence variation within the small ribosomal subunit. rrsH-bearing ribosomes affect the expression of functionally coherent gene sets and alter the levels of the RpoS sigma factor, the master regulator of the general stress response. These impacts are associated with phenotypic changes in antibiotic sensitivity, biofilm formation, and cell motility and are regulated by stress response proteins, RelA and RelE, as well as the metabolic enzyme and virulence-associated protein, AdhE. These findings establish that endogenously encoded, naturally occurring rRNA sequence variation can modulate ribosome function, central aspects of gene expression regulation, and cellular physiology. Most organisms encode multiple, distinct copies of rRNA genes, rendering the composition of the ribosome pool intrinsically heterogeneous. Here, Kurylo et al. show that nutrient limitation in E. coli upregulates the expression of ribosomes bearing conserved sequence variation in 16S rRNA that can regulate gene expression and phenotype.
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影响因子：
16
作者：
Culviner PH;Laub MT
通讯作者：
Laub MT
严格控制核糖体依赖性激活。
影响因子：
64.8
作者：
Brown A;Fernández IS;Gordiyenko Y;Ramakrishnan V
通讯作者：
Ramakrishnan V
影响因子：
46.9
作者：
Cho, Byung-Kwan;Zengler, Karsten;Qiu, Yu;Park, Young Seoub;Knight, Eric M.;Barrett, Christian L.;Gao, Yuan;Palsson, Bernhard O.
通讯作者：
Palsson, Bernhard O.
影响因子：
11.4
作者：
CONDON, C;PHILIPS, J;SQUIRES, CL
通讯作者：
SQUIRES, CL
影响因子：
4.4
作者：
Cox, Juergen;Neuhauser, Nadin;Mann, Matthias
通讯作者：
Mann, Matthias