Enhancement of grain number per spike by RNA interference of cytokinin oxidase 2 gene in bread wheat.
Enhancement of grain number per spike by RNA interference of cytokinin oxidase 2 gene in bread wheat.
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RNA干扰面包小麦细胞分裂素氧化酶2基因提高每穗粒数
DOI：
10.1186/s41065-018-0071-7
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发表时间：
2018
期刊：
影响因子：
2.7
通讯作者：
Li G
中科院分区：
文献类型：
--
作者：
Li Y;Song G;Gao J;Zhang S;Zhang R;Li W;Chen M;Liu M;Xia X;Risacher T;Li G
本研究旨在验证CKX基因对小麦籽粒数的调控作用。我们构建了TaCKX2.4的RNA干扰表达载体，并将其转化到面包小麦品系NB 1中。利用Southern杂交技术筛选单拷贝的转基因植株。采用实时定量PCR（qRT-PCR）检测TaCKX2.4基因的表达。验证了TaCKX2.4基因的表达量与籽粒数的关系。共获得20个阳性独立事件。选择来自5个事件的纯合株系，每个都具有单拷贝的转化基因，以评估T3代中TaCKX2.4的表达和每穗粒数。与对照NB 1相比，T3系JW 39 -3A、JW 1 -2B、JW 1 -1A和JW 5 -1A的穗粒数分别增加12.6%、8.3%、6.5%和5.8%。我们的研究表明，TaCKX2.4的表达水平与每穗粒数呈负相关，表明TaCKX2.4的表达降低会增加小麦的每穗粒数。
This study aimed to validate the function of CKX gene on grain numbers in wheat. we constructed and transformed a RNA interference expression vector of TaCKX2.4 in bread wheat line NB1. Southern blotting analysis was used to select transgenic plants with single copy. The expression of TaCKX2.4 gene was estimated by Quantitative real-time PCR (qRT-PCR) analysis. Finally, the relation between expression of TaCKX2.4 gene and grain numbers was validated. Totally, 20 positive independent events were obtained. Homozygous lines from 5 events with a single copy of transformed gene each were selected to evaluate the expression of TaCKX2.4 and grain numbers per spike in T3 generation. Compared with the control NB1, the average grain numbers per spike significantly increased by 12.6%, 8.3%, 6.5% and 5.8% in the T3 lines JW39-3A, JW1-2B, JW1-1A and JW5-1A, respectively. Our study indicated that the expression level of TaCKX2.4 was negatively correlated with the grain number per spike, indicating that the reduced expression of TaCKX2.4 increased grain numbers per spike in wheat.
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影响因子：
2.6
作者：
Chen, Junbo;Liang, Yun;Luo, Peigao
通讯作者：
Luo, Peigao
DOI：
10.1007/bf00293832
发表时间：
1991-12-01
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
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通讯作者：
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4.2
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影响因子：
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作者：
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影响因子：
3.7
作者：
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通讯作者：
Allakhverdiev, Suleyman I.