What was the set of ubiquitin and ubiquitin-like conjugating enzymes in the eukaryote common ancestor?
What was the set of ubiquitin and ubiquitin-like conjugating enzymes in the eukaryote common ancestor?
复制标题
DOI：
10.1007/s00239-009-9225-6
复制
发表时间：
2009-06
影响因子：
3.9
通讯作者：
Andres, Christian R.
中科院分区：
文献类型：
--
作者：
Michelle, Caroline;Vourc'h, Patrick;Mignon, Laurence;Andres, Christian R.
泛素 (Ub) 结合酶 (E2) 是蛋白质泛素化或 Ub 样修饰的关键酶。我们在七个物种（智人、小家鼠、果蝇、秀丽隐杆线虫、粟酒裂殖酵母、酿酒酵母和拟南芥）中搜索了属于 E2 酶超家族的所有蛋白质，以识别家族并重建每个家族的系统发育。我们对 207 个基因的系统发育分析使我们在人类基因组中定义了 17 个 E2 家族，其中有 37 个 E2 基因。 E2 细分为四类与系统发育树不对应。序列特征 HPN（组氨酸 - 脯氨酸 - 天冬酰胺），后跟 16 个（最多 29 个）氨基酸的色氨酸残基，是高度保守的。当存在时，在距 HPN C 末端 7 至 8 个氨基酸处发现了活性半胱氨酸。二级结构的特征是典型的α/β折叠。只有家族 10 偏离了共同的组织，因为这些蛋白质缺乏酶活性。家族 7 在 β 链 1 和 2 之间有一个插入；家族 3、5 和 14 在活性半胱氨酸和保守色氨酸之间有插入。这些蛋白质的三维数据凸显了核心结构域的强大结构保守性。我们的分析表明，原始真核生物祖先拥有多种 E2 酶，从而强调了 Ub 途径的重要性。对 E2 酶的全面概述强调了这个超家族的多样性和进化，并有助于阐明命名法和真正的直系同源性。更好地了解这些酶的功能对于破译几种人类疾病是必要的。本文的在线版本 (doi:10.1007/s00239-009-9225-6) 包含补充材料，可供授权用户使用。
Ubiquitin (Ub)-conjugating enzymes (E2) are key enzymes in ubiquitination or Ub-like modifications of proteins. We searched for all proteins belonging to the E2 enzyme super-family in seven species (Homo sapiens, Mus musculus, Drosophila melanogaster, Caenorhabditis elegans, Schizosaccharomyces pombe, Saccharomyces cerevisiae, and Arabidopsis thaliana) to identify families and to reconstruct each family’s phylogeny. Our phylogenetic analysis of 207 genes led us to define 17 E2 families, with 37 E2 genes, in the human genome. The subdivision of E2 into four classes did not correspond to the phylogenetic tree. The sequence signature HPN (histidine–proline–asparagine), followed by a tryptophan residue at 16 (up to 29) amino acids, was highly conserved. When present, the active cysteine was found 7 to 8 amino acids from the C-terminal end of HPN. The secondary structures were characterized by a canonical alpha/beta fold. Only family 10 deviated from the common organization because the proteins were devoid of enzymatic activity. Family 7 had an insertion between beta strands 1 and 2; families 3, 5 and 14 had an insertion between the active cysteine and the conserved tryptophan. The three-dimensional data of these proteins highlight a strong structural conservation of the core domain. Our analysis shows that the primitive eukaryote ancestor possessed a diversified set of E2 enzymes, thus emphasizing the importance of the Ub pathway. This comprehensive overview of E2 enzymes emphasizes the diversity and evolution of this superfamily and helps clarify the nomenclature and true orthologies. A better understanding of the functions of these enzymes is necessary to decipher several human diseases. The online version of this article (doi:10.1007/s00239-009-9225-6) contains supplementary material, which is available to authorized users.
登录
查看更多内容
影响因子：
12.3
作者：
通讯作者：
--
影响因子：
64.5
作者：
Koegl, M;Hoppe, T;Jentsch, S
通讯作者：
Jentsch, S
影响因子：
2
作者：
Cottee, Pauline A.;El-Osta, Youssef G. Abs;Gasser, Robin B.
通讯作者：
Gasser, Robin B.
影响因子：
7
作者：
Mushegian, AR;Garey, JR;Liu, LX
通讯作者：
Liu, LX
DOI：
10.1107/s0907444998002480
发表时间：
1998-09-01
期刊：
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
影响因子：
--
作者：
Giraud, MF;Desterro, JMP;Naismith, JH
通讯作者：
Naismith, JH