Quantitative analysis of mechanical force required for cell extrusion in zebrafish embryonic epithelia.
Quantitative analysis of mechanical force required for cell extrusion in zebrafish embryonic epithelia.
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DOI：
10.1242/bio.027847
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发表时间：
2017-10-15
期刊：
影响因子：
2.4
通讯作者：
Matsui T
中科院分区：
文献类型：
--
作者：
Yamada S;Iino T;Bessho Y;Hosokawa Y;Matsui T
当上皮层中的细胞由于内在或外在原因而受损时，它们通过从层中挤出而被消除。细胞挤出，这是需要维持组织的完整性，是收缩的肌动球蛋白环的结果，如分子/细胞生物学实验和数值模拟所证明的。然而，由于缺乏合适的直接测量系统，尚未进行肌动球蛋白收缩的定量评价。在这项研究中，我们开发了一种新的方法，使用飞秒激光来量化的收缩力的肌动球蛋白环在斑马鱼胚胎上皮细胞挤出。在该系统中，斑马鱼胚胎中的上皮细胞首先被直接飞秒激光照射损伤。接下来，飞秒激光诱导的冲击力被加载到肌动球蛋白环上，并且收缩力被量化为以kPa为单位的压力。我们发现，当收缩力稍微减弱时，细胞挤出被延迟，这表明相对较小的力足以驱动细胞挤出。因此，我们的方法是适合于相对定量评价的力学动力学过程中的细胞挤压，原则上，该方法适用于类似的现象，在不同的组织和器官的各种物种。总结：在这项研究中，开发了一种新型的体内力量化系统，该系统成功地估计了从斑马鱼胚胎上皮中挤出垂死细胞所需的力的大小。
When cells in epithelial sheets are damaged by intrinsic or extrinsic causes, they are eliminated by extrusion from the sheet. Cell extrusion, which is required for maintenance of tissue integrity, is the consequence of contraction of actomyosin rings, as demonstrated by both molecular/cellular biological experimentation and numerical simulation. However, quantitative evaluation of actomyosin contraction has not been performed because of the lack of a suitable direct measurement system. In this study, we developed a new method using a femtosecond laser to quantify the contraction force of the actomyosin ring during cell extrusion in zebrafish embryonic epithelia. In this system, an epithelial cell in zebrafish embryo is first damaged by direct femtosecond laser irradiation. Next, a femtosecond laser-induced impulsive force is loaded onto the actomyosin ring, and the contraction force is quantified to be on the order of kPa as a unit of pressure. We found that cell extrusion was delayed when the contraction force was slightly attenuated, suggesting that a relatively small force is sufficient to drive cell extrusion. Thus, our method is suitable for the relative quantitative evaluation of mechanical dynamics in the process of cell extrusion, and in principle the method is applicable to similar phenomena in different tissues and organs of various species. Summary: In this study a novel in vivo force quantification system was developed, which succeeded in estimating the magnitude of force required for extrusion of a dying cell from zebrafish embryonic epithelia.
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影响因子：
2.3
作者：
Iino, Takanori;Hosokawa, Yoichiroh
通讯作者：
Hosokawa, Yoichiroh
影响因子：
56.9
作者：
Behrndt, Martin;Salbreux, Guillaume;Heisenberg, Carl-Philipp
通讯作者：
Heisenberg, Carl-Philipp
影响因子：
4
作者：
Kuipers, Dorothy;Mehonic, Aida;Gale, Jonathan E.
通讯作者：
Gale, Jonathan E.
影响因子：
21.3
作者：
Balaban, NQ;Schwarz, US;Geiger, B
通讯作者：
Geiger, B
DOI：
10.1083/jcb.200609116
发表时间：
2007-01-01
期刊：
The Journal of cell biology
影响因子：
--
作者：
Tamada M;Perez TD;Nelson WJ;Sheetz MP
通讯作者：
Sheetz MP