Two distinct modes of myosin assembly and dynamics during epithelial wound closure.
Two distinct modes of myosin assembly and dynamics during epithelial wound closure.
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DOI：
10.1083/jcb.200609116
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发表时间：
2007-01-01
期刊：
影响因子：
--
通讯作者：
Sheetz MP
中科院分区：
文献类型：
--
作者：
Tamada M;Perez TD;Nelson WJ;Sheetz MP
在胚胎发生和伤口愈合过程中，肌动球蛋白的收缩促进上皮片的密封；然而，人们对其机制知之甚少。在激光消融犬Madin-Darby肾细胞单层损伤后，我们从肌球蛋白分布的延时图像中观察到伤口愈合的不同步骤。受伤后，肌动蛋白和肌凝蛋白II调节轻链在两个位置积聚：(1)在紧结处附近的环上，(2)在细胞底部的纤维中，在伤口上延伸的膜上。rho激酶的活性是肌凝蛋白环组装所必需的，肌凝蛋白II的活性是肌收缩所必需的，而不是基底膜伸展所必需的。随着收缩，肌凝蛋白环与ZO-1和rho激酶一起向基膜移动。因此，我们认为，在伤口闭合过程中，紧密连接作为肌动球蛋白环的附着点，而rho激酶是定位和激活收缩环所必需的。
Actomyosin contraction powers the sealing of epithelial sheets during embryogenesis and wound closure; however, the mechanisms are poorly understood. After laser ablation wounding of Madin–Darby canine kidney cell monolayers, we observed distinct steps in wound closure from time-lapse images of myosin distribution during resealing. Immediately upon wounding, actin and myosin II regulatory light chain accumulated at two locations: (1) in a ring adjacent to the tight junction that circumscribed the wound and (2) in fibers at the base of the cell in membranes extending over the wound site. Rho-kinase activity was required for assembly of the myosin ring, and myosin II activity was required for contraction but not for basal membrane extension. As it contracted, the myosin ring moved toward the basal membrane with ZO-1 and Rho-kinase. Thus, we suggest that tight junctions serve as attachment points for the actomyosin ring during wound closure and that Rho-kinase is required for localization and activation of the contractile ring.
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影响因子：
64.8
作者：
MARTIN, P;LEWIS, J
通讯作者：
LEWIS, J
影响因子：
5.3
作者：
Sin, WC;Chen, XQ;Lim, L
通讯作者：
Lim, L
影响因子：
3.3
作者：
Vogelmann, R;Nelson, WJ
通讯作者：
Nelson, WJ
DOI：
10.1083/jcb.146.2.439
发表时间：
1999-07-26
期刊：
The Journal of cell biology
影响因子：
--
作者：
Shelton CA;Carter JC;Ellis GC;Bowerman B
通讯作者：
Bowerman B
DOI：
10.1083/jcb.149.2.471
发表时间：
2000-04-17
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kiehart DP;Galbraith CG;Edwards KA;Rickoll WL;Montague RA
通讯作者：
Montague RA