Discriminating between latent and active tuberculosis with multiple biomarker responses.
Discriminating between latent and active tuberculosis with multiple biomarker responses.
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DOI：
10.1016/j.tube.2011.02.006
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发表时间：
2011-05
期刊：
影响因子：
--
通讯作者：
Stout JE
中科院分区：
文献类型：
--
作者：
Frahm M;Goswami ND;Owzar K;Hecker E;Mosher A;Cadogan E;Nahid P;Ferrari G;Stout JE
我们试图鉴定对结核特异性抗原的生物标志物应答，其可以1）改善结核感染的诊断和2）允许区分活动性和潜伏性感染。对70例活动性结核（N=12）、潜伏性结核（N=32）或无结核感染证据（N=26）受试者进行了评价。我们使用Luminex Multiplexed Bead Array平台，使用Quantiferon® Gold In-Tube试剂盒，在过夜刺激后同时评估全血样品上清液中的25种生物标志物。我们将对刺激的反应定义为对合并的结核抗原和阴性对照的反应之间的差异（在个体患者内）。与未感染组相比，结核病感染（活动性或潜伏性）受试者的IP-10反应显着更高（p <0.0001）。在25个参数中，发现活动性结核组中IL-15和MCP-1的表达水平显著高于潜伏性结核组（分别为p = 0.0006和0.0030）。当结合使用时，IL-15和MCP-1准确地识别了83%的活动性感染和88%的潜伏性感染。在这项研究中，IL-15和MCP-1反应的组合在区分活动性结核病患者和潜伏性结核病患者方面是准确的。
We sought to identify biomarker responses to tuberculosis specific antigens which could 1) improve the diagnosis of tuberculosis infection and 2) allow the differentiation of active and latent infections. Seventy subjects with active tuberculosis (N=12), latent tuberculosis (N=32), or no evidence of tuberculosis infection (N=26) were evaluated. We used the Luminex Multiplexed Bead Array platform to simultaneously evaluate 25 biomarkers in the supernatant of whole blood samples following overnight stimulation using the Quantiferon® Gold In-Tube kit. We defined the response to stimulation as the difference (within an individual patient) between the response to the pooled tuberculosis antigens and the negative control. IP-10 response was significantly higher in tuberculosis-infected (active or latent) subjects compared to the uninfected group (p <0.0001). Among the 25 parameters, expression levels of IL-15 and MCP-1 were found to be significantly higher in the active tuberculosis group compared to the latent tuberculosis group (p = 0.0006 and 0.0030, respectively). When combined, IL-15 and MCP-1 accurately identified 83% of active and 88% of latent infections. The combination of IL-15 and MCP-1 responses was accurate in distinguishing persons with active tuberculosis from persons with latent tuberculosis in this study.
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IP-10，MCP-1，MCP-2，MCP-3和IL-1RA在整个血液基的T细胞测定法中作为结核分枝杆菌感染的生物标志物具有希望。
影响因子：
1.8
作者：
Ruhwald M;Bjerregaard-Andersen M;Rabna P;Eugen-Olsen J;Ravn P
通讯作者：
Ravn P
DOI：
10.1084/jem.20050126
发表时间：
2005-12-19
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Flores-Villanueva PO;Ruiz-Morales JA;Song CH;Flores LM;Jo EK;Montaño M;Barnes PF;Selman M;Granados J
通讯作者：
Granados J
影响因子：
3.7
作者：
Ganachari, Malathesha;Ruiz-Morales, Jorge A.;Flores-Villanueva, Pedro O.
通讯作者：
Flores-Villanueva, Pedro O.
影响因子：
120.7
作者：
Dye, C;Scheele, S;Raviglione, RC
通讯作者：
Raviglione, RC
DOI：
10.1198/106186006x133933
发表时间：
2006-09-01
影响因子：
2.4
作者：
Hothorn, Torsten;Hornik, Kurt;Zeileis, Achim
通讯作者：
Zeileis, Achim