The ribosome assembly factor Nop53 has a structural role in the formation of nuclear pre-60S intermediates, affecting late maturation events.
The ribosome assembly factor Nop53 has a structural role in the formation of nuclear pre-60S intermediates, affecting late maturation events.
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DOI：
10.1093/nar/gkab494
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发表时间：
2021-07-09
影响因子：
14.9
通讯作者：
Oliveira CC
中科院分区：
文献类型：
--
作者：
Bagatelli FFM;de Luna Vitorino FN;da Cunha JPC;Oliveira CC
真核核糖体的生物发生是一个复杂的过程，在这个过程中，核糖体蛋白与pre-rRNA在加工和折叠过程中组装在一起。数以百计的组装因子（AF）是必需的，并瞬时招募，以协助顺序重塑事件。其中最复杂的是5.8S和25S rnas之间的内部转录间隔器2 （ITS2）的逐步去除，它与5个AFs一起构成了60s前的“脚”。在从核核向核质过渡的过程中，Nop53取代了位于足部基础的Erb1，并招募RNA外泌体进行ITS2切割和足部拆卸。本文综合分析了Nop53的募集对60s前植株组成变化的影响。我们发现，与缺乏外泌体相互作用基序的Nop53突变体不同，Nop53的缺失不仅会导致60年代前中间产物中未加工脚的保留，还会影响从核核状态E粒子到后续核阶段的转变。此外，我们发现Nop53缺失会导致Yvh1募集等晚成熟事件的损害。根据最近描述的60年代前的低温电镜结构，我们的研究结果为Nop53重新排列和稳定足部界面以协助Nog2颗粒形成的结构作用提供了生化证据。
Eukaryotic ribosome biogenesis is an elaborate process during which ribosomal proteins assemble with the pre-rRNA while it is being processed and folded. Hundreds of assembly factors (AF) are required and transiently recruited to assist the sequential remodeling events. One of the most intricate ones is the stepwise removal of the internal transcribed spacer 2 (ITS2), between the 5.8S and 25S rRNAs, that constitutes together with five AFs the pre-60S ‘foot’. In the transition from nucleolus to nucleoplasm, Nop53 replaces Erb1 at the basis of the foot and recruits the RNA exosome for the ITS2 cleavage and foot disassembly. Here we comprehensively analyze the impact of Nop53 recruitment on the pre-60S compositional changes. We show that depletion of Nop53, different from nop53 mutants lacking the exosome-interacting motif, not only causes retention of the unprocessed foot in late pre-60S intermediates but also affects the transition from nucleolar state E particle to subsequent nuclear stages. Additionally, we reveal that Nop53 depletion causes the impairment of late maturation events such as Yvh1 recruitment. In light of recently described pre-60S cryo-EM structures, our results provide biochemical evidence for the structural role of Nop53 rearranging and stabilizing the foot interface to assist the Nog2 particle formation.
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影响因子：
16
作者：
Albert, Benjamin;Knight, Britta;Shore, David
通讯作者：
Shore, David
DOI：
10.1261/rna.062901.117
发表时间：
2017-12
期刊：
RNA (New York, N.Y.)
影响因子：
--
作者：
Falk S;Tants JN;Basquin J;Thoms M;Hurt E;Sattler M;Conti E
通讯作者：
Conti E
影响因子：
10.5
作者：
Gamalinda M;Ohmayer U;Jakovljevic J;Kumcuoglu B;Woolford J;Mbom B;Lin L;Woolford JL Jr
通讯作者：
Woolford JL Jr
影响因子：
16.8
作者：
Barrio-Garcia, Clara;Thoms, Matthias;Hurt, Ed
通讯作者：
Hurt, Ed
影响因子：
4.8
作者：
Cepeda, Leidy Paola P.;Bagatelli, Felipe F. M.;Oliveira, Carla C.
通讯作者：
Oliveira, Carla C.