Fructose-bisphophate aldolase exhibits functional roles between carbon metabolism and the hrp system in rice pathogen Xanthomonas oryzae pv. oryzicola.
Fructose-bisphophate aldolase exhibits functional roles between carbon metabolism and the hrp system in rice pathogen Xanthomonas oryzae pv. oryzicola.
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果糖二磷酸醛缩酶在水稻病原菌米黄单胞菌的碳代谢和 hrp 系统之间发挥功能作用。
DOI：
10.1371/journal.pone.0031855
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Chen GY
中科院分区：
文献类型：
--
作者：
Guo W;Zou LF;Li YR;Cui YP;Ji ZY;Cai LL;Zou HS;Hutchins WC;Yang CH;Chen GY
果糖-二磷酸醛缩酶（FbaB）是一种在生物体内参与糖酵解和糖异生的酶。对水稻白叶枯病菌（Xanthomonas oryzae pv.）的一个独特的fbaB基因进行了诱变。水稻细菌性条斑病的病原菌oryzicola不仅不能利用丙酮酸和苹果酸生长，而且在以果糖为唯一碳源时，其生长受到抑制，胞外多糖（EPS）的产生量减少，细菌的毒力和生长受到损害。有趣的是，fbaB启动子含有一个不完全的PIP盒（植物诱导型启动子）（TTCGT-N9-TTCGT）。fbaB的表达受关键hrp调控因子HrpG和HrpX的负调控。PIP盒中的碱基替换改变了fbaB与级联反应的调节。此外，还发现fbaB在X. oryzaepv.稻生RS 105菌株在植物中而不是在富含营养的培养基中是可诱导的。除hrp-hrc-hpa基因外，fbaB基因缺失后，hrpG和hrpX基因的表达受到抑制，而hrcC、hrpE和hpa 3基因的表达增强。hrcC、hrpE或hpa 3的突变降低了病原体获得丙酮酸和苹果酸的能力。此外，在RΔfbaB突变体中，由于fbaB的反式存在，细菌的毒力、在植物中的生长和EPS产量完全恢复到野生型水平。oryzaepv. Oryzicola通过一种未知调节因子在协调HRP基因表达中起关键作用。
Fructose-bisphophate aldolase (FbaB), is an enzyme in glycolysis and gluconeogenesis in living organisms. The mutagenesis in a unique fbaB gene of Xanthomonas oryzae pv. oryzicola, the causal agent of rice bacterial leaf streak, led the pathogen not only unable to use pyruvate and malate for growth and delayed its growth when fructose was used as the sole carbon source, but also reduced extracellular polysaccharide (EPS) production and impaired bacterial virulence and growth in rice. Intriguingly, the fbaB promoter contains an imperfect PIP-box (plant-inducible promoter) (TTCGT-N9-TTCGT). The expression of fbaB was negatively regulated by a key hrp regulatory HrpG and HrpX cascade. Base substitution in the PIP-box altered the regulation of fbaB with the cascade. Furthermore, the expression of fbaB in X. oryzae pv. oryzicola RS105 strain was inducible in planta rather than in a nutrient-rich medium. Except other hrp-hrc-hpa genes, the expression of hrpG and hrpX was repressed and the transcripts of hrcC, hrpE and hpa3 were enhanced when fbaB was deleted. The mutation in hrcC, hrpE or hpa3 reduced the ability of the pathogen to acquire pyruvate and malate. In addition, bacterial virulence and growth in planta and EPS production in RΔfbaB mutant were completely restored to the wild-type level by the presence of fbaB in trans. This is the first report to demonstrate that carbohydrates, assimilated by X. oryzae pv. oryzicola, play critical roles in coordinating hrp gene expression through a yet unknown regulator.
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