Tactile Mapping System
Tactile Mapping System
复制标题
触觉测绘系统
作者：
Tomonori Oie;Hisato Suzuki;T. Fukuda;Y. Murayama;S. Omata;K. Kanda;Y. Nakayama
目的研究触觉映射系统（TMS）在组织或器官表面弹性分布映射方面具有高度的空间精度。方法采用小口径猪动脉（直径：~ 3mm）的圆周切面，在聚氨酯薄片上涂覆14 μm厚的明胶水凝胶，进行区域微图型制备弹性测试图，该弹性测试图具有线和空间配置，用于弹性分布图的绘制。采用直径为5或12 μm、空间间隔为1 ~ 5 μm、压痕深度为4 μm的探针，同时对生理盐水中的表面形貌和弹性进行了研究。结果在最小探针直径和最小探针运动空间间隔的条件下，在水下获得了<5 μm的TMS空间分辨率。利用TMS对猪动脉平面周切（厚度~0.5 mm）进行表面弹性分布图绘制，在<2 μm的空间分辨率下，可以清晰区分血管壁的同心层，包括富胶原层和富弹性蛋白层。结论TMS是一种在<2 μm空间分辨率下测量维管组织表面弹性分布的简单、廉价的方法。TMS能够分析生理盐水下组织样本的复杂结构。
Objective We demonstrated that the tactile mapping system (TMS) has a high degree of spatial precision in the distribution mapping of surface elasticity of tissues or organs. Methods Samples used were a circumferential section of a small-caliber porcine artery (diameter: ~3 mm) and an elasticity test pattern with a line and space configuration for the distribution mapping of elasticity, prepared by regional micropatterning of a 14-μm thick gelatin hydrogel coating on a polyurethane sheet. Surface topography and elasticity in normal saline were simultaneously investigated by TMS using a probe with a diameter of 5 or 12 μm, a spatial interval of 1 to 5 μm, and an indentation depth of 4 μm. Results In the test pattern, a spatial resolution in TMS of <5 μm was acquired under water with a minimal probe diameter and spatial interval of the probe movement. TMS was used for the distribution mapping of surface elasticity in a flat, circumferential section (thickness: ~0.5 mm) of a porcine artery, and the concentric layers of the vascular wall, including the collagen-rich and elastin-rich layers, could be clearly differentiated in terms of surface elasticity at the spatial resolution of <2 μm. Conclusions TMS is a simple and inexpensive technique for the distribution mapping of the surface elasticity in vascular tissues at the spatial resolution <2 μm. TMS has the ability to analyze a complex structure of the tissue samples under normal saline.
影响因子：
3.4
作者：
Aikaterini Zoumi;Xiao Lu;G. Kassab;B. Tromberg
通讯作者：
Aikaterini Zoumi;Xiao Lu;G. Kassab;B. Tromberg
DOI：
10.1007/978-1-59745-443-8_18
发表时间：
2007
期刊：
Methods in molecular medicine
影响因子：
--
作者：
D. Schmidt;A. Mol;J. Kelm;S. Hoerstrup
通讯作者：
D. Schmidt;A. Mol;J. Kelm;S. Hoerstrup