Phenotypic memory in Bacillus subtilis links dormancy entry and exit by a spore quantity-quality tradeoff.
Phenotypic memory in Bacillus subtilis links dormancy entry and exit by a spore quantity-quality tradeoff.
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DOI：
10.1038/s41467-017-02477-1
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发表时间：
2018-01-04
影响因子：
16.6
通讯作者：
Bischofs IB
中科院分区：
文献类型：
--
作者：
Mutlu A;Trauth S;Ziesack M;Nagler K;Bergeest JP;Rohr K;Becker N;Höfer T;Bischofs IB
一些细菌，例如枯草芽孢杆菌，通过形成休眠孢子来抵御饥饿，当营养物质可用时，孢子就会复活。尽管孢子形成和孢子复兴共同决定了波动环境中的生存，但它们之间的关系尚不清楚。在这里，我们证明这两个过程通过表型“记忆”联系在一起，这种记忆是由分子从营养细胞转移到孢子中而产生的。通过使用荧光报告基因对单个枯草芽孢杆菌细胞的生命史进行成像，我们证明孢子形成时间控制营养诱导的孢子复兴。丙氨酸脱氢酶有助于孢子记忆并控制丙氨酸诱导的生长，从而将孢子的复兴能力与其祖细胞的基因表达和生长历史结合起来。理论分析和信号突变体表现出改变的孢子形成时间的实验支持这样的假设：这种内在产生的记忆导致孢子数量和孢子质量之间的权衡，这可能推动复杂微生物性状的出现。 枯草芽孢杆菌通过形成休眠孢子来抵御饥饿，当营养物质可用时，孢子就会复活。在这里，穆特鲁等人。研究表明，孢子形成时间通过表型“记忆”控制孢子复活，这种表型“记忆”是由代谢酶从营养细胞转移到孢子中而产生的。
Some bacteria, such as Bacillus subtilis, withstand starvation by forming dormant spores that revive when nutrients become available. Although sporulation and spore revival jointly determine survival in fluctuating environments, the relationship between them has been unclear. Here we show that these two processes are linked by a phenotypic “memory” that arises from a carry-over of molecules from the vegetative cell into the spore. By imaging life histories of individual B. subtilis cells using fluorescent reporters, we demonstrate that sporulation timing controls nutrient-induced spore revival. Alanine dehydrogenase contributes to spore memory and controls alanine-induced outgrowth, thereby coupling a spore’s revival capacity to the gene expression and growth history of its progenitors. A theoretical analysis, and experiments with signaling mutants exhibiting altered sporulation timing, support the hypothesis that such an intrinsically generated memory leads to a tradeoff between spore quantity and spore quality, which could drive the emergence of complex microbial traits. Bacillus subtilis withstands starvation by forming dormant spores that revive when nutrients become available. Here, Mutlu et al. show that sporulation timing controls spore revival through a phenotypic ‘memory’ that arises from the carry-over of a metabolic enzyme from the vegetative cell into the spore.
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影响因子：
11.3
作者：
Higgins D;Dworkin J
通讯作者：
Dworkin J
DOI：
10.1073/pnas.1002499107
发表时间：
2010-05-04
影响因子：
11.1
作者：
Chastanet, Arnaud;Vitkup, Dennis;Losick, Richard M.
通讯作者：
Losick, Richard M.
影响因子：
9.8
作者：
Even-Tov E;Bendori SO;Valastyan J;Ke X;Pollak S;Bareia T;Ben-Zion I;Bassler BL;Eldar A
通讯作者：
Eldar A
影响因子：
48
作者：
Gibson, Daniel G.;Young, Lei;Smith, Hamilton O.
通讯作者：
Smith, Hamilton O.
影响因子：
5
作者：
Chen, Zhen-Min;Li, Qing;Chen, Xiang-Dong
通讯作者：
Chen, Xiang-Dong