Birdsong "transcriptomics": neurochemical specializations of the oscine song system.
Birdsong "transcriptomics": neurochemical specializations of the oscine song system.
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Birdsong“转录组学”：Oscine歌曲系统的神经化学专业。
DOI：
10.1371/journal.pone.0003440
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发表时间：
2008
期刊：
影响因子：
3.7
通讯作者：
Mello, Claudio V.
中科院分区：
文献类型：
--
作者：
Lovell, Peter V.;Clayton, David F.;Replogle, Kirstin L.;Mello, Claudio V.
发声学习是一种罕见而复杂的行为特征，是人类口语习得的基础。在鸣禽中，声音的学习和产生依赖于一组被称为“歌唱系统”的专门的大脑核。利用高通量功能基因组学，我们已经鉴定出成年斑马雀HVC（鸣叫系统的关键节点）的约200个新的分子标记。这些标记清楚地将HVC与HVC所属的一般苍白区区分开来，从而代表了该歌核的分子专门化。生物信息学分析表明，几种主要的神经细胞功能和特定的生化途径是HVC转录调控的靶点，包括：1)细胞-细胞和细胞-底物相互作用（例如，钙粘蛋白/连环蛋白介导的粘附连接，胶原介导的局点粘附，信号素-神经磷脂/丛蛋白轴突引导途径）；2)细胞兴奋性（如钾通道亚家族、胆碱能和血清素能受体、神经肽和神经肽受体）；3)信号转导（如钙调节蛋白、g蛋白相关信号的调节因子）；4)细胞增殖/死亡、迁移和分化（如tgf - β /BMP和p53通路）；5)基因表达的调控（候选类维生素a和类固醇靶点，染色质/核仁组织的调节剂）。调控的总体方向表明，与细胞稳定性相关的过程在成年HVC中得到增强，而增殖、生长和可塑性在很大程度上受到抑制，这与该鸣禽在成年期的鸣叫声基本稳定的观察结果一致。我们的研究代表了一个最全面的分子遗传特征的脑核涉及一个复杂的学习行为在脊椎动物。这些数据表明了歌唱系统的药理学和遗传操作的许多目标，并为可能在歌唱行为和/或声乐学习调节中发挥作用的机制提供了新的见解。
Vocal learning is a rare and complex behavioral trait that serves as a basis for the acquisition of human spoken language. In songbirds, vocal learning and production depend on a set of specialized brain nuclei known as the song system. Using high-throughput functional genomics we have identified ∼200 novel molecular markers of adult zebra finch HVC, a key node of the song system. These markers clearly differentiate HVC from the general pallial region to which HVC belongs, and thus represent molecular specializations of this song nucleus. Bioinformatics analysis reveals that several major neuronal cell functions and specific biochemical pathways are the targets of transcriptional regulation in HVC, including: 1) cell-cell and cell-substrate interactions (e.g., cadherin/catenin-mediated adherens junctions, collagen-mediated focal adhesions, and semaphorin-neuropilin/plexin axon guidance pathways); 2) cell excitability (e.g., potassium channel subfamilies, cholinergic and serotonergic receptors, neuropeptides and neuropeptide receptors); 3) signal transduction (e.g., calcium regulatory proteins, regulators of G-protein-related signaling); 4) cell proliferation/death, migration and differentiation (e.g., TGF-beta/BMP and p53 pathways); and 5) regulation of gene expression (candidate retinoid and steroid targets, modulators of chromatin/nucleolar organization). The overall direction of regulation suggest that processes related to cell stability are enhanced, whereas proliferation, growth and plasticity are largely suppressed in adult HVC, consistent with the observation that song in this songbird species is mostly stable in adulthood. Our study represents one of the most comprehensive molecular genetic characterizations of a brain nucleus involved in a complex learned behavior in a vertebrate. The data indicate numerous targets for pharmacological and genetic manipulations of the song system, and provide novel insights into mechanisms that might play a role in the regulation of song behavior and/or vocal learning.
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DOI：
10.1002/neu.10070
发表时间：
2002-07-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Bailey, DJ;Rosebush, JC;Wade, J
通讯作者：
Wade, J
影响因子：
1.7
作者：
Jarvis, ED;Schwabl, H;Mello, CV
通讯作者：
Mello, CV
影响因子：
2.8
作者：
Lomax, MI;Huang, L;Altschuler, RA
通讯作者：
Altschuler, RA
影响因子：
4.4
作者：
Malyala, A;Pattee, P;Ronnekleiv, OK
通讯作者：
Ronnekleiv, OK
DOI：
10.1073/pnas.0701619104
发表时间：
2007-04-17
影响因子：
11.1
作者：
Li, XiaoChing;Wang, Xiu-Jie;Gaasterland, Terry
通讯作者：
Gaasterland, Terry