Occurrence of Serratia marcescens Carrying bla(IMP-26) and mcr-9 in Southern China: New Insights in the Evolution of Megaplasmid IMP-26.
Occurrence of Serratia marcescens Carrying bla(IMP-26) and mcr-9 in Southern China: New Insights in the Evolution of Megaplasmid IMP-26.
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DOI：
10.3390/antibiotics11070869
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发表时间：
2022-06-28
影响因子：
4.8
通讯作者：
Chen, Dingqiang
中科院分区：
文献类型：
--
作者：
Zhong, Yuxia;Liu, Wanting;Yuan, Peibo;Yang, Ling;Xu, Zhenbo;Chen, Dingqiang
关键词：
The spread of multidrug-resistant enterobacteria strains has posed a significant concern in public health, especially when the strain harbors metallo-beta-lactamase (MBL)-encoding and mobilized colistin resistance (mcr) genes as such genetic components potentially mediate multidrug resistance. Here we report an IncHI2/2A plasmid carrying blaIMP-26 and mcr-9 in multidrug-resistant Serratia marcescens human isolates YL4. Antimicrobial susceptibility testing was performed by the broth microdilution method. According to the results, S. marcescens YL4 was resistant to several antimicrobials, including β-lactams, fluorquinolones, sulfanilamide, glycylcycline, and aminoglycosides, except for amikacin. To investigate the plasmid further, we conducted whole-genome sequencing and sequence analysis. As shown, S. marcescens YL4 possessed a circular chromosome with 5,171,477 bp length and two plasmids, pYL4.1 (321,744 bp) and pYL4.2 (46,771 bp). Importantly, sharing high similarity with plasmids pZHZJ1 and pIMP-26, pYL4.1 has an IncHI2/2A backbone holding a variable region containing blaIMP-26, mcr-9, and two copies of blaTEM-1B. After comprehensively comparing relevant plasmids, we proposed an evolutionary pathway originating from ancestor pZHZJ1. Then, via an acquisition of the mcr-9 element and a few recombination events, this plasmid eventually evolved into pYL4.1 and pIMP-26 through two different pathways. In addition, the phage-like plasmid pYL4.2 also carried a blaTEM-1B gene. Remarkably, this study first identified a multidrug-resistant S. marcescens strain co-harboring blaIMP-26 and mcr-9 on a megaplasmid pYL4.1 and also included a proposed evolutionary pathway of epidemic megaplasmids carrying blaIMP-26.
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影响因子：
9.4
作者：
Karina Merkier, Andrea;Cecilia Rodriguez, Mara;Centron, Daniela
通讯作者：
Centron, Daniela
影响因子：
6.9
作者：
Al Jarousha, A. M. Kh.;El Qouqa, I. A.;Al Afifi, A. S.
通讯作者：
Al Afifi, A. S.
影响因子：
4.8
作者：
Chavda, Kalyan D.;Westblade, Lars F.;Kreiswirth, Barry N.
通讯作者：
Kreiswirth, Barry N.
DOI：
10.4161/mge.24774
发表时间：
2013-03-01
期刊：
Mobile genetic elements
影响因子：
--
作者：
Harms K;Starikova I;Johnsen PJ
通讯作者：
Johnsen PJ
影响因子：
3.9
作者：
Gogry FA;Siddiqui MT;Sultan I;Haq QMR
通讯作者：
Haq QMR