Glutamate formed from lignoceric acid by rat brain preparation in the presence of pyridine nucleotide and cytosolic factors: a brain-specific oxidation of very long chain fatty acids.
Glutamate formed from lignoceric acid by rat brain preparation in the presence of pyridine nucleotide and cytosolic factors: a brain-specific oxidation of very long chain fatty acids.
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在吡啶核苷酸和胞质因子存在下,通过大鼠大脑制备,由二十四烷酸形成谷氨酸:极长链脂肪酸的大脑特异性氧化。
DOI:
10.1021/bi00508a039
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发表时间:
1981
期刊:
影响因子:
2.9
通讯作者:
Kishimoto,Y
中科院分区:
文献类型:
--
作者:
Uda,M;Singh,I;Kishimoto,Y
Masayuki Uda, J Inderjit Singh, and Yasuo Kishimoto* abstract: When [l-14C] lignoceric acid was incubated with a rat brain particulate fraction in the presence of heat-stable and heat-labile factors and NADPH, considerable radioactivity was found in the water-soluble fraction. These conditions were similar to those for the synthesis of cerebronic acid (ahydroxylation) and ceramide synthesis. The water-soluble product was purified to apparent homogeneity by combining gel filtration, steric exclusion high-performance liquid chromatography, and CM-Sepharose chromatography. The pu-rified material was identified as glutamic acid by an NMR spectrum, high-performanceliquid chromatography with three different columns, ninhydrin test, and thin-layer chromatog-raphy-radioautography. The incorporation of radioactivity into glutamate from lignoceric acid absolutely required heat-stable and heat-labile factors. NADPH could be replaced
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影响因子:
3.9
作者:
I. Singh;Y. Kishimoto
通讯作者:
Y. Kishimoto
影响因子:
4.7
作者:
D. Seidel;G. Nowoczek;H. Jatzkewitz
通讯作者:
H. Jatzkewitz
影响因子:
4.8
作者:
I. Singh;Y. Kishimoto
通讯作者:
Y. Kishimoto
影响因子:
4.7
作者:
H. Akanuma;Y. Kishimoto
通讯作者:
Y. Kishimoto
DOI:
10.1016/s0021-9258(18)81113-9
发表时间:
1963
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
J. Mead;G. Levis
通讯作者:
G. Levis