The paradoxical dynamism of marrow stem cells: considerations of stem cells, niches, and microvesicles.
The paradoxical dynamism of marrow stem cells: considerations of stem cells, niches, and microvesicles.
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DOI：
10.1007/s12015-008-9036-y
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发表时间：
2008-09
影响因子：
4.8
通讯作者：
Aliotta, Jason M.
中科院分区：
文献类型：
--
作者：
Quesenberry, Peter J.;Aliotta, Jason M.
关键词：
骨髓干细胞调控是一个复杂而灵活的系统。它已被假定，该系统本质上是分层的，但最近的数据表明，在祖细胞/干细胞水平的系统可能代表一个连续体与可逆的表型改变发生的干细胞过境细胞周期。短期和长期的植入，在体内和体外分化，基因表达，和祖细胞数量都被发现与细胞周期可逆地变化。本质上，干细胞似乎显示出可变的潜力，可能是基于转录因子的访问，因为它们通过细胞周期进行。干细胞调控的另一个关键组成部分是微环境，即所谓的生态位。我们认为，不仅有几个独特的小生境细胞，但各种各样的小生境细胞不断改变表型，适当地与连续的干细胞表型相互作用。调控系统的第三个组成部分是细胞间遗传信息的微泡转移。我们已经证明，在微泡从肺转移到骨髓细胞后，骨髓细胞可以表达肺上皮细胞的遗传表型。组织特异性mRNA的类似转移发生在肝脏、脑和心脏到骨髓细胞之间。因此，通过细胞之间的微泡转移，似乎存在细胞的连续遗传调节。我们提出，有一个互动的三角形维恩图与不断变化的干细胞相互作用的影响，不断变化的领域，都是由微泡遗传信息的转移调制。
Marrow stem cell regulation represents a complex and flexible system. It has been assumed that the system was intrinsically hierarchical in nature, but recent data has indicated that at the progenitor/stem cell level the system may represent a continuum with reversible alterations in phenotype occurring as the stem cells transit cell cycle. Short and long-term engraftment, in vivo and in vitro differentiation, gene expression, and progenitor numbers have all been found to vary reversibly with cell cycle. In essence, the stem cells appear to show variable potential, probably based on transcription factor access, as they proceed through cell cycle. Another critical component of the stem cell regulation is the microenvironment, so-called niches. We propose that there are not just several unique niche cells, but a wide variety of niche cells which continually change phenotype to appropriately interact with the continuum of stem cell phenotypes. A third component of the regulatory system is microvesicle transfer of genetic information between cells. We have shown that marrow cells can express the genetic phenotype of pulmonary epithelial cells after microvesicle transfer from lung to marrow cells. Similar transfers of tissue specific mRNA occur between liver, brain, and heart to marrow cells. Thus, there would appear to be a continuous genetic modulation of cells through microvesicle transfer between cells. We propose that there is an interactive triangulated Venn diagram with continuously changing stem cells interacting with continuously changing areas of influence, both being modulated by transfer of genetic information by microvesicles.
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DOI：
10.1089/152581602321080574
发表时间：
2002-12-01
期刊：
JOURNAL OF HEMATOTHERAPY & STEM CELL RESEARCH
影响因子：
--
作者：
Cerny, J;Dooner, M;Quesenberry, P
通讯作者：
Quesenberry, P
影响因子：
20.3
作者：
Arber, C;BitMansour, A;Brown, JMY
通讯作者：
Brown, JMY
影响因子：
64.5
作者：
Adolfsson, J;Månsson, R;Jacobsen, SEW
通讯作者：
Jacobsen, SEW
影响因子：
2.6
作者：
Aliotta, JM;Keaney, P;Quesenberry, PJ
通讯作者：
Quesenberry, PJ
DOI：
10.1038/icb.1966.28
发表时间：
1966-01-01
期刊：
AUSTRALIAN JOURNAL OF EXPERIMENTAL BIOLOGY AND MEDICAL SCIENCE
影响因子：
--
作者：
BRADLEY, TR;METCALF, D
通讯作者：
METCALF, D