The biomarkers changes in serum and the correlation with quantitative MRI markers by histopathologic evaluation of the cartilage in surgically-induced osteoarthritis rabbit model.
The biomarkers changes in serum and the correlation with quantitative MRI markers by histopathologic evaluation of the cartilage in surgically-induced osteoarthritis rabbit model.
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通过对手术诱导的骨关节炎兔模型中的软骨进行组织病理学评估，观察血清中生物标志物的变化以及与定量 MRI 标志物的相关性。
DOI：
10.1371/journal.pone.0124717
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yao W
中科院分区：
文献类型：
--
作者：
Zuo H;Jiang L;Qu N;Wang J;Cui X;Yao W
通过对兔骨关节炎（OA）模型的软骨组织病理学评价，探讨血清生物标志物的变化及其与定量MRI指标的相关性。使用36只成年新西兰兔。将18只家兔随机分为6组，每组10只。另18只家兔随机分为6组，每组10只，作为对照组。在手术后的多个时间点，通过ELISA分析血清中的BMP-2、CTX-II和COMP水平，并进行定量MRI。组织学检查采用HE染色，并评估Mankin评分。使用配对样本T检验比较OA组与对照组中生化生物标志物和成像标志物的变化。分析MRI定量标记物与生物标记物和Mankin评分的相关性。采用非参数wilcoxon符号秩检验进行Mankin评分分析。骨形态发生蛋白-2在术后不同时间均升高，与对照组比较差异有显著性（P均<0.001）。CTX-II水平在手术后的几个时间段显著升高，包括W2、W8、W12、W16和W20（分别为P=0.019、0.004、0.007、<0.001和0.016），但在W 4没有升高（P=0.764）。从W2到W20，OA组与对照组之间观察到COMP水平的显著差异（分别为P<0.001、P <0.001、P <0.001、P <0.001、P =0.002和P =0.004）。T2值在术后第8周增加，并且在OA组和对照组之间存在显著差异（分别为P=0.001、<0.001、<0.001和<0.001）。T2* 值从W2到W20增加，并且在对照组和OA组之间具有显著差异（分别为P=0.002、P =0.001、P <0.001、P <0.001、P =0.001和P <0.001）。T2值与BMP-2、CTX-Ⅱ有显著相关性（P<0.001，P =0.014），与COMP无显著相关性（P=0.305）。T2* 值与BMP-2、CTX-II和COMP的相关性均显著（P值分别为0.043、0.005和0.025）。T2值与Mankin评分呈正相关（P<0.001）。通过多时间点分析血清生物标志物和影像学标志物与组织学结果的相关性，BMP-2、CTX-II和COMP联合T2和T2* 可用于潜在地反映和监测OA进展。
To investigate the biomarkers change in serum and the correlation with quantitative MRI markers by histopathologic evaluation of the cartilage in surgically-induced osteoarthritis(OA) rabbit model. Thirty-six mature New Zealand rabbits were used. Eighteen rabbits were divided into six groups randomly and equally and subjected to surgery using the improved Hulth method. The other eighteen rabbits were also allocated into six groups randomly and equally which served as the control. At multiple time points after surgery, the BMP-2, CTX-II and COMP levels in the serum were analyzed by ELISA, and quantitative MRI was performed. Histopathology was examined with HE, and Mankin scores were assessed. The changes in the biochemical biomarkers and imaging markers in the OA groups were compared with those in the control groups using paired-samples T tests. The correlation of quantitative MRI markers with biomarkers and Mankin scores were analyzed. The analysis of Mankin scores was conducted with non-parametric wilcoxon signed rank tests. The BMP-2 levels were increased at various times after surgery, and significant differences were observed between the OA and control groups(all the P values <0.001). CTX-II levels were significantly elevated at several intervals after surgery, including W2, W8, W12, W16 and W20(P=0.019, 0.004, 0.007, <0.001 and 0.016 respectively), but not at W4(P=0.764). Significant differences in the COMP levels from W2 to W20 were observed between the OA and the control groups(P<0.001, <0.001, <0.001, <0.001,=0.002 and =0.004 respectively). The T2 values increased at W8 post-surgery and were significantly different between the OA and control groups(P=0.001, <0.001, <0.001 and <0.001 respectively). T2* values increased from W2 to W20 and were significantly different between the control and OA groups(P=0.002, =0.001, <0.001, <0.001, =0.001 and <0.001 respectively). T2 values had significant correlation with BMP-2 and CTX-II(P<0.001 and =0.014), except COMP(P=0.305)., while the correlation of T2* values with BMP-2, CTX-II and COMP was significant(P=0.043, 0.005 and 0.025 respectively). In addition, a positive correlation of T2 values and Mankin scores was observed(P<0.001). With the relevance of the multiple time point analysis of the serum biomarkers and imaging markers compared with histological findings, BMP-2, CTX-II and COMP combined with T2 and T2* can be used to reflect and monitor OA progression potentially.
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影响因子：
27.4
作者：
Davidson, E. N. Blaney;Vitters, E. L.;van den Berg, W. B.
通讯作者：
van den Berg, W. B.
DOI：
10.3109/17453677008991540
发表时间：
1970-01-01
期刊：
ACTA ORTHOPAEDICA SCANDINAVICA
影响因子：
--
作者：
HULTH, A;LINDBERG, L;TELHAG, H
通讯作者：
TELHAG, H
影响因子：
7
作者：
Bakker, AC;van de Loo, FAJ;van den Berg, WB
通讯作者：
van den Berg, WB
影响因子：
--
作者：
EHRLICH, MG;HOULE, PA;MANKIN, HJ
通讯作者：
MANKIN, HJ
影响因子：
7
作者：
Gelse, K.;Muehle, C.;Schneider, H.
通讯作者：
Schneider, H.