Expression of endoplasmic reticulum stress protein in rabbit condyle cartilage following anterior disk displacement
Expression of endoplasmic reticulum stress protein in rabbit condyle cartilage following anterior disk displacement
复制标题
椎间盘前移兔髁突软骨内质网应激蛋白的表达
DOI：
10.1111/jop.12715
复制
发表时间：
2018-07
影响因子：
3.3
通讯作者：
Xu T
中科院分区：
文献类型：
--
作者：
Xu Ting;Yao Hua;Wang Guohua;Xu Ting;Yao Hua;Wang Guohua;Gu Zhiyuan;Wu Huiling;Xu T
目的
关节盘前移位（ADD）是颞下颌关节紊乱病（TMD）最常见的一种亚型，其发生与软骨细胞凋亡有关。然而，触发细胞凋亡的信号通路仍然是未知的。本研究的目的是研究ADD后兔髁突软骨中内质网（ER）应激相关蛋白的表达。
方法
将60只健康成年家兔随机分为实验组和假手术对照组（n = 12）。实验兔右侧颞下颌关节行手术ADD。通过免疫组织化学、实时定量PCR和Western印迹分析评估软骨中ER应激相关蛋白C/EBP同源蛋白（CHOP）、葡萄糖调节蛋白78（GRP 78）、裂解的caspase-3和caspase-12的产生。
结果
我们的研究结果表明，CHOP，GRP 78，裂解caspase-3，caspase-12的表达显着增加沿着在ADD后软骨退行性变化。
结论
这些结果表明ER应激途径在ADD软骨中被激活，并可能促进TMD的发展。
OBJECTIVE
Anterior disk displacement (ADD) is a most common subtype of temporomandibular disorders (TMD), which is promoted by chondrocytes apoptosis. However, the signaling pathways that trigger apoptosis are still unknown. The aim of this study was to investigate the expression of endoplasmic reticulum (ER) stress-related proteins in the condylar cartilage of rabbits following ADD.
METHODS
Sixty healthy adult rabbits were randomly assigned to the experimental and sham-operated control groups (n = 12). The experimental rabbits were subjected to surgical ADD in the right temporomandibular joints. The production of ER stress-related proteins C/EBP homologous protein (CHOP), glucose-regulated protein 78 (GRP78), cleaved caspase-3, and caspase-12 in cartilage was evaluated by immunohistochemistry, quantitative real-time PCR, and Western blot analysis.
RESULTS
Our results showed that the expression of CHOP, GRP78, cleaved caspase-3, and caspase-12 increased significantly along with degenerative changes in cartilage after ADD.
CONCLUSION
These results indicate that the ER stress pathway is activated in ADD cartilage and might promote the development of TMD.
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影响因子：
2.7
作者：
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通讯作者：
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影响因子：
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作者：
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