Pseudogene INTS6P1 regulates its cognate gene INTS6 through competitive binding of miR-17-5p in hepatocellular carcinoma.
Pseudogene INTS6P1 regulates its cognate gene INTS6 through competitive binding of miR-17-5p in hepatocellular carcinoma.
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DOI：
10.18632/oncotarget.3290
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发表时间：
2015-03-20
期刊：
影响因子：
--
通讯作者：
Selaru FM
中科院分区：
文献类型：
--
作者：
Peng H;Ishida M;Li L;Saito A;Kamiya A;Hamilton JP;Fu R;Olaru AV;An F;Popescu I;Iacob R;Dima S;Alexandrescu ST;Grigorie R;Nastase A;Berindan-Neagoe I;Tomuleasa C;Graur F;Zaharia F;Torbenson MS;Mezey E;Lu M;Selaru FM
抑癌基因及其假基因的复杂调控在肝细胞癌（HCC）的发病机制中起着关键作用。然而，假基因在肝癌发病机制中的作用仍不完全清楚。本研究通过全基因组表达谱芯片技术鉴定了肝癌中的抑癌基因INTS 6及其假基因INTS 6P 1。此外，功能研究-包括生长曲线，细胞死亡，迁移测定和体内研究-验证了INTS 6和INTS 6P 1在HCC中的肿瘤抑制作用。最后，机制实验表明，INTS 6和INTS 6P 1通过竞争oncomiR-17- 5 p而被间接调节。综上所述，这些发现表明INTS 6P 1和INTS 6通过竞争oncomiR-17- 5 p发挥肿瘤抑制作用。我们对这一调节回路的研究揭示了肝癌发生机制的新见解。
The complex regulation of tumor suppressive gene and its pseudogenes play key roles in the pathogenesis of hepatocellular cancer (HCC). However, the roles played by pseudogenes in the pathogenesis of HCC are still incompletely elucidated. This study identifies the putative tumor suppressor INTS6 and its pseudogene INTS6P1 in HCC through the whole genome microarray expression. Furthermore, the functional studies – include growth curves, cell death, migration assays and in vivo studies – verify the tumor suppressive roles of INTS6 and INTS6P1 in HCC. Finally, the mechanistic experiments indicate that INTS6 and INTS6P1 are reciprocally regulated through competition for oncomiR-17-5p. Taken together, these findings demonstrate INTS6P1 and INTS6 exert the tumor suppressive roles through competing for oncomiR-17-5p. Our investigation of this regulatory circuit reveals novel insights into the underlying mechanisms of hepatocarcinogenesis.
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影响因子：
8
作者：
Braconi, C.;Kogure, T.;Valeri, N.;Huang, N.;Nuovo, G.;Costinean, S.;Negrini, M.;Miotto, E.;Croce, C. M.;Patel, T.
通讯作者：
Patel, T.
在致癌 BRAF 诱导的黑色素瘤小鼠模型中体内鉴定肿瘤抑制性 PTEN ceRNA。
影响因子：
64.5
作者：
Karreth FA;Tay Y;Perna D;Ala U;Tan SM;Rust AG;DeNicola G;Webster KA;Weiss D;Perez-Mancera PA;Krauthammer M;Halaban R;Provero P;Adams DJ;Tuveson DA;Pandolfi PP
通讯作者：
Pandolfi PP
CERNA假设：隐藏RNA语言的Rosetta石头？
影响因子：
64.5
作者：
Salmena L;Poliseno L;Tay Y;Kats L;Pandolfi PP
通讯作者：
Pandolfi PP
影响因子：
64.5
作者：
Kalyana-Sundaram S;Kumar-Sinha C;Shankar S;Robinson DR;Wu YM;Cao X;Asangani IA;Kothari V;Prensner JR;Lonigro RJ;Iyer MK;Barrette T;Shanmugam A;Dhanasekaran SM;Palanisamy N;Chinnaiyan AM
通讯作者：
Chinnaiyan AM
影响因子：
5.8
作者：
Filleur S;Hirsch J;Wille A;Schön M;Sell C;Shearer MH;Nelius T;Wieland I
通讯作者：
Wieland I