Phthalate esters used as plasticizers in packed red blood cell storage bags may lead to progressive toxin exposure and the release of pro-inflammatory cytokines.
Phthalate esters used as plasticizers in packed red blood cell storage bags may lead to progressive toxin exposure and the release of pro-inflammatory cytokines.
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DOI：
10.4161/oxim.2.3.8608
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发表时间：
2009-07
影响因子：
--
通讯作者：
Bar-Or D
中科院分区：
文献类型：
--
作者：
Rael LT;Bar-Or R;Ambruso DR;Mains CW;Slone DS;Craun ML;Bar-Or D
邻苯二甲酸酯（PE）是用于软化pvc基医疗器械的增塑剂。PE是最丰富的人造污染物，会增加患过敏性呼吸道疾病或恶性肿瘤的风险。评估了储存过程中捐献红细胞（PRBC）中PE的浸出情况。对含有CPD/AS-1 （ADSOL）缓冲液的PRBC输液袋进行分析。在储存第1天和第42天采集样品。采用液相色谱-质谱联用（LCMS）法测定了邻苯二甲酸二（2-乙基己基）酯（DEHP）和邻苯二甲酸单（2-乙基己基）酯（MEHP）两种PE。采用标准ELISA法检测白细胞介素-8 （IL-8）。DEHP从第1天的34.3µM（±20.0 SD）显著增加到第42天的433.2µM（±131.2 SD），增加12.6倍。同样，MEHP从第1天的3.7µM（±2.8 SD）显著增加到第42天的74.0µM（±19.1 SD），增加了20.2倍。DEHP和MEHP还能增加人脐静脉内皮细胞（HUVEC） IL-8的释放。旧单位的PRBC的输血可能导致PE的积累，可能导致炎症和其他影响。由于创伤患者具有较低的酯酶活性（负责代谢PE的酶），因此PE的代谢降低可能会加剧这种积累。由PE引起的氧化应激的影响被认为是由较老的PRBC引起的炎症增加的潜在机制。
Phthalate esters (PE's) are plasticizers used to soften PVC-based medical devices. PE's are the most abundant man-made pollutants and increase the risk of developing an allergic respiratory disease or a malignancy. The leaching of PE's in donated packed red blood cells (PRBC) during storage was assessed. PRBC transfusion bags containing CPD/AS-1 (ADSOL) buffer were analyzed. Samples were collected on storage day 1 and day 42. Two PE's, di-(2-ethylhexyl) phthalate (DEHP) and mono-(2-ethylhexyl) phthalate (MEHP), were measured by liquid chromatography coupled to mass spectrometry (LCMS). Interleukin-8 (IL-8) was measured by standard ELISA techniques. DEHP significantly increased from 34.3 µM (±20.0 SD) on day 1 to 433.2 µM (±131.2 SD) on day 42, a 12.6-fold increase. Similarly, MEHP significantly increased from 3.7 µM (±2.8 SD) on day 1 to 74.0 µM (±19.1 SD) on day 42, a 20.2-fold increase. Also, DEHP and MEHP increased the release of IL-8 from human umbilical vein endothelial cells (HUVEC). The transfusion of older units of PRBC could lead to an accumulation of PE's possibly resulting in inflammation and other effects. This accumulation could be exacerbated due to the decreased metabolism of PE's since trauma patients have a lower esterase activity, the enzymes responsible for metabolizing PE's. The effect of oxidative stress caused by PE's is discussed as a potential mechanism for increases in inflammation caused by older units of PRBC.
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DOI：
10.1016/j.bbrc.2007.03.182
发表时间：
2007-06-01
影响因子：
3.1
作者：
Rael, Leonard T.;Rao, Nagaraja K. R.;Bar-Or, David
通讯作者：
Bar-Or, David
影响因子：
10.4
作者：
ROCK, G;LABOW, RS;TOCCHI, M
通讯作者：
TOCCHI, M
影响因子：
3.5
作者：
Larsen, Soren Thor;Nielsen, Gunnar Damgard
通讯作者：
Nielsen, Gunnar Damgard
影响因子：
10.4
作者：
GRIFFITHS, WC;CAMARA, PD;GENTILE, J
通讯作者：
GENTILE, J
影响因子：
3.5
作者：
Hong, Yun-Chul;Park, Eun-Young;Ha, Eun-Hee
通讯作者：
Ha, Eun-Hee