CD4 T cells in the rheumatoid joint are oligoclonally activated and change during the course of disease.
CD4 T cells in the rheumatoid joint are oligoclonally activated and change during the course of disease.
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类风湿关节中的 CD4 T 细胞被寡克隆激活并在疾病过程中发生变化。
DOI：
10.1136/ard.54.4.314
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发表时间：
1995
影响因子：
27.4
通讯作者：
A. So
中科院分区：
文献类型：
--
作者：
H. Khazaei;C. Lunardi;A. So
目的--评估类风湿关节中CD 4 T细胞利用T细胞受体（TCR）的性质。方法--对3名患者从滑液CD 4 T细胞中分离的TCR β链mRNA的连接（NDJ）区进行测序，以确定是否存在特定序列的寡克隆扩增。对两名患者进行了纵向研究，以确定这些序列是否随时间发生变化。结果--在每个患者的TCR转录本测序中发现了许多显性克隆型。在纵向研究的两名患者中，随着时间的推移检测到不同的优势克隆型。在研究期间，没有单一的克隆型持续占主导地位。结论：TCR的使用模式显示类风湿关节内有多个寡克隆扩增的CD 4 T细胞。随着时间的推移，关节内克隆型的变化表明，不同的抗原可能能够在类风湿性疾病的过程中引起滑膜炎症。
OBJECTIVE--To assess the nature of T cell receptor (TCR) utilisation by CD4 T cells in the rheumatoid joint. METHODS--Sequencing of the joining (NDJ) region of TCR beta chain mRNA isolated from synovial fluid CD4 T cells was performed in three patients in order to determine if oligoclonal expansion of particular sequences was present. Two patients were studied longitudinally to determine if these sequences changed over time. RESULTS--A number of dominant clonotypes were found within the TCR transcripts sequenced in each patient. In the two patients who were studied longitudinally, different dominant clonotypes were detected over time. No single clonotype was persistently dominant during the period of study. CONCLUSIONS--The pattern of TCR usage showed multiple oligoclonally expanded CD4 T cells within the rheumatoid joint. The change in clonotypes within the joint over time suggests that different antigens may be able to elicit synovial inflammation during the course of rheumatoid disease.
影响因子：
56.9
作者：
PALIARD, X;WEST, SG;KOTZIN, BL
通讯作者：
KOTZIN, BL