A global DNA methylation and gene expression analysis of early human B-cell development reveals a demethylation signature and transcription factor network.
A global DNA methylation and gene expression analysis of early human B-cell development reveals a demethylation signature and transcription factor network.
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DOI：
10.1093/nar/gks957
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发表时间：
2012-12
影响因子：
14.9
通讯作者：
Wiemels JL
中科院分区：
文献类型：
--
作者：
Lee ST;Xiao Y;Muench MO;Xiao J;Fomin ME;Wiencke JK;Zheng S;Dou X;de Smith A;Chokkalingam A;Buffler P;Ma X;Wiemels JL
The epigenetic changes during B-cell development relevant to both normal function and hematologic malignancy are incompletely understood. We examined DNA methylation and RNA expression status during early B-cell development by sorting multiple replicates of four separate stages of pre-B cells derived from normal human fetal bone marrow and applied high-dimension DNA methylation scanning and expression arrays. Features of promoter and gene body DNA methylation were strongly correlated with RNA expression in multipotent progenitors (MPPs) both in a static state and throughout differentiation. As MPPs commit to pre-B cells, a predominantly demethylating phenotype ensues, with 79% of the 2966 differentially methylated regions observed involving demethylation. Demethylation events were more often gene body associated rather than promoter associated; predominantly located outside of CpG islands; and closely associated with EBF1, E2F, PAX5 and other functional transcription factor (TF) sites related to B-cell development. Such demethylation events were accompanied by TF occupancy. After commitment, DNA methylation changes appeared to play a smaller role in B-cell development. We identified a distinct development-dependent demethylation signature which has gene expression regulatory properties for pre-B cells, and provide a catalog reference for the epigenetic changes that occur in pre-B-cell leukemia and other B-cell-related diseases.
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影响因子：
46.9
作者：
Ball, Madeleine P.;Li, Jin Billy;Gao, Yuan;Lee, Je-Hyuk;LeProust, Emily M.;Park, In-Hyun;Xie, Bin;Daley, George Q.;Church, George M.
通讯作者：
Church, George M.
影响因子：
14.9
作者：
Calvanese V;Fernández AF;Urdinguio RG;Suárez-Alvarez B;Mangas C;Pérez-García V;Bueno C;Montes R;Ramos-Mejía V;Martínez-Camblor P;Ferrero C;Assenov Y;Bock C;Menendez P;Carrera AC;Lopez-Larrea C;Fraga MF
通讯作者：
Fraga MF
影响因子：
32.4
作者：
Decker, Thorsten;di Magliano, Marina Pasca;Busslinger, Meinrad
通讯作者：
Busslinger, Meinrad
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
6.5
作者：
Golfier, F;Bárcena, A;Muench, MO
通讯作者：
Muench, MO