Invasive characteristics of human prostatic epithelial cells: understanding the metastatic process.
Invasive characteristics of human prostatic epithelial cells: understanding the metastatic process.
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DOI：
10.1038/sj.bjc.6602325
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发表时间：
2005-02-14
影响因子：
8.8
通讯作者：
中科院分区：
文献类型：
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作者：
前列腺癌具有通过尚未表征的机制转移到骨髓基质（BMS）的倾向。我们已经定义了一系列的共培养模型的入侵，模拟血液/BMS的边界，并允许阐明的信号和力学的跨内皮细胞迁移内的复杂的骨髓环境。共聚焦显微镜显示前列腺上皮细胞特异性结合骨髓内皮细胞-内皮细胞连接并启动内皮细胞收缩。跨内皮迁移通过上皮细胞伪足过程进行，在232±43分钟后发生完全上皮迁移。基质衍生因子-1（SDF-1）/CXCR 4信号转导诱导PC-3穿过基底膜侵入，尽管侵入水平比对BMS（P=0.0007）或骨髓内皮细胞（P=0.004）的侵入低3.5倍。10 μM SDF-1抑制剂T140可完全抑制最大SDF-1侵袭信号传导。然而，10 μM T140仅分别使对BMS和骨髓内皮细胞的侵袭减少59%（P=0.001）和29%（P=0.011）。这项研究强调了研究信号分子和/或抑制剂的潜在作用的必要性，不仅在单细胞模型中，而且在模拟骨髓复杂环境的共培养模型中。
Prostate cancer has a predilection to metastasise to the bone marrow stroma (BMS) by an as yet uncharacterised mechanism. We have defined a series of coculture models of invasion, which simulate the blood/BMS boundary and allow the elucidation of the signalling and mechanics of trans-endothelial migration within the complex bone marrow environment. Confocal microscopy shows that prostate epithelial cells bind specifically to bone marrow endothelial-to-endothelial cell junctions and initiate endothelial cell retraction. Trans-endothelial migration proceeds via an epithelial cell pseudopodial process, with complete epithelial migration occurring after 232±43 min. Stromal-derived factor-1 (SDF-1)/CXCR4 signalling induced PC-3 to invade across a basement membrane although the level of invasion was 3.5-fold less than invasion towards BMS (P=0.0007) or bone marrow endothelial cells (P=0.004). Maximal SDF-1 signalling of invasion was completely inhibited by 10 μM of the SDF-1 inhibitor T140. However, 10 μM T140 only reduced invasion towards BMS and bone marrow endothelial cells by 59% (P=0.001) and 29% (P=0.011), respectively. This study highlights the need to examine the potential roles of signalling molecules and/or inhibitors, not just in single-cell models but in coculture models that mimic the complex environment of the bone marrow.
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巨核细胞对基质细胞衍生因子1（SDF-1）的跨内皮迁移增强了血小板的形成。
影响因子：
15.3
作者：
Hamada, T;Mohle, R;Hesselgesser, J;Hoxie, J;Nachman, R L;Moore, M A;Rafii, S
通讯作者：
Rafii, S
影响因子：
4.8
作者：
CURRY, FE
通讯作者：
CURRY, FE
影响因子：
8
作者：
Burger, M;Glodek, A;Burger, JA
通讯作者：
Burger, JA
影响因子：
64.8
作者：
Müller, A;Homey, B;Zlotnik, A
通讯作者：
Zlotnik, A
DOI：
10.1084/jem.185.1.111
发表时间：
1997-01-06
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Aiuti A;Webb IJ;Bleul C;Springer T;Gutierrez-Ramos JC
通讯作者：
Gutierrez-Ramos JC