Conditional, genetic disruption of ciliary neurotrophic factor receptors reveals a role in adult motor neuron survival.
Conditional, genetic disruption of ciliary neurotrophic factor receptors reveals a role in adult motor neuron survival.
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DOI：
10.1111/j.1460-9568.2008.06298.x
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发表时间：
2008-06
期刊：
影响因子：
--
通讯作者：
Maclennan AJ
中科院分区：
文献类型：
--
作者：
Lee N;Robitz R;Zurbrugg RJ;Karpman AM;Mahler AM;Cronier SA;Vesey R;Spearry RP;Zolotukhin S;Maclennan AJ
Indirect evidence suggests that endogenous ciliary neurotrophic factor (CNTF) receptor signaling can promote motor neuron (MN) survival in the adult. If so, proper targeting of this signaling may selectively counteract the effects of adult MN diseases. However, direct evidence for CNTF receptor involvement in adult MN survival is lacking, presumably because the unconditional blockade of the mouse CNTF receptor in vivo [through genetic disruption of the essential CNTF receptor α (CNTFRα) gene] leads to uniform perinatal death of the mice. To overcome this limitation, we have developed a method to selectively disrupt CNTF receptor function in a targeted subset of adult MNs that are not required for survival. A ‘floxed CNTFRα’ mouse line was generated and characterized. In addition, an adeno-associated virus (AAV) vector that drives Cre recombinase (Cre) expression was constructed and shown, with reporter mouse lines, to selectively excise floxed genes in facial MNs following its stereotaxic injection into the facial motor nucleus. Adult floxed CNTFRα mice were then injected with the AAV-Cre vector to excise the CNTFRα gene in the targeted MNs. The resulting data indicate that adult CNTF receptor signaling, likely by the MNs themselves, can play an essential role in MN survival. The data further indicate that this role is independent of any developmental contributions CNTF receptor signaling makes to MN survival or function.
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影响因子：
64.5
作者：
DECHIARA, TM;VEJSADA, R;YANCOPOULOS, GD
通讯作者：
YANCOPOULOS, GD
影响因子：
25
作者：
Pun, S;Santos, AF;Caroni, P
通讯作者：
Caroni, P
影响因子：
56.9
作者：
DAVIS, S;ALDRICH, TH;YANCOPOULOS, GD
通讯作者：
YANCOPOULOS, GD
影响因子：
4.2
作者：
Bartlett, JS;Samulski, RJ;McCown, TJ
通讯作者：
McCown, TJ
影响因子：
64.8
作者：
LI, M;SENDTNER, M;SMITH, A
通讯作者：
SMITH, A