Genomic adaptations of Campylobacter jejuni to long-term human colonization.
Genomic adaptations of Campylobacter jejuni to long-term human colonization.
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DOI：
10.1186/s13099-021-00469-7
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发表时间：
2021-12-10
期刊：
影响因子：
4.2
通讯作者：
Benschop J
中科院分区：
文献类型：
--
作者：
Bloomfield SJ;Midwinter AC;Biggs PJ;French NP;Marshall JC;Hayman DTS;Carter PE;Mather AE;Fayaz A;Thornley C;Kelly DJ;Benschop J
弯曲杆菌是从人类和动物的胃肠道以及他们生活在世界各地的环境中分离出来的一种细菌。弯曲杆菌通过其基因含量和表达的变化来适应新的环境，但人们对它们如何适应长期的人类殖民知之甚少。在这项研究中，来自新西兰患者的31个分离物和来自英国患者的22个属于空肠弯曲菌序列45型(ST45)的分离物的基因组与来自其他来源的209个ST45基因组进行了比较，以确定弯曲菌适应人类长期定植的机制。此外，新西兰患者使用16S rRNA元编码研究了他们的微生物区系，并使用生化测试分析了他们的炎症和免疫抑制水平，以确定弯曲杆菌如何适应不断变化的胃肠道。有一些证据表明，长期的定植导致了基因组的退化，但更多的证据表明，弯曲杆菌是通过参与细胞运动、信号转导和主要外膜蛋白(MOMP)的非同义单核苷酸多态(SNPs)和移码基因的积累来适应的。新西兰患者的微生物群、炎症和免疫抑制在五个月内也表现出相当大的变化，从该患者身上收集的弯曲杆菌可分为两个亚群，其比例与胃肠道炎症的程度相关。这项研究展示了基因组学、系统发生学、16S rRNA变位编码和生化标记如何提供关于弯曲杆菌如何适应人类宿主内不断变化的环境的洞察。这项研究还表明，长期的人类定居选择了与细胞运动、信号转导和MOMP有关的弯曲杆菌基因的变化；弯曲杆菌遗传上不同的亚群进化以适应不断变化的胃肠道环境。网上版载有补充材料，可在10.1186/s13099-021-00469-7查阅。
Campylobacter is a genus of bacteria that has been isolated from the gastrointestinal tract of humans and animals, and the environments they inhabit around the world. Campylobacter adapt to new environments by changes in their gene content and expression, but little is known about how they adapt to long-term human colonization. In this study, the genomes of 31 isolates from a New Zealand patient and 22 isolates from a United Kingdom patient belonging to Campylobacter jejuni sequence type 45 (ST45) were compared with 209 ST45 genomes from other sources to identify the mechanisms by which Campylobacter adapts to long-term human colonization. In addition, the New Zealand patient had their microbiota investigated using 16S rRNA metabarcoding, and their level of inflammation and immunosuppression analyzed using biochemical tests, to determine how Campylobacter adapts to a changing gastrointestinal tract. There was some evidence that long-term colonization led to genome degradation, but more evidence that Campylobacter adapted through the accumulation of non-synonymous single nucleotide polymorphisms (SNPs) and frameshifts in genes involved in cell motility, signal transduction and the major outer membrane protein (MOMP). The New Zealand patient also displayed considerable variation in their microbiome, inflammation and immunosuppression over five months, and the Campylobacter collected from this patient could be divided into two subpopulations, the proportion of which correlated with the amount of gastrointestinal inflammation. This study demonstrates how genomics, phylogenetics, 16S rRNA metabarcoding and biochemical markers can provide insight into how Campylobacter adapts to changing environments within human hosts. This study also demonstrates that long-term human colonization selects for changes in Campylobacter genes involved in cell motility, signal transduction and the MOMP; and that genetically distinct subpopulations of Campylobacter evolve to adapt to the changing gastrointestinal environment. The online version contains supplementary material available at 10.1186/s13099-021-00469-7.
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影响因子：
3.4
作者：
Bouckaert RR;Drummond AJ
通讯作者：
Drummond AJ
通才弯曲杆菌菌株中快速的宿主切换侵蚀了追踪人类感染的信号。
DOI：
10.1038/ismej.2015.149
发表时间：
2016-03
期刊：
The ISME journal
影响因子：
--
作者：
Dearlove BL;Cody AJ;Pascoe B;Méric G;Wilson DJ;Sheppard SK
通讯作者：
Sheppard SK
影响因子：
4.5
作者：
Feliziani S;Marvig RL;Luján AM;Moyano AJ;Di Rienzo JA;Krogh Johansen H;Molin S;Smania AM
通讯作者：
Smania AM
影响因子：
3.9
作者：
Dunn, Steven J.;Pascoe, Ben;Manning, Georgina
通讯作者：
Manning, Georgina
影响因子：
1.7
作者：
Bankevich, Anton;Nurk, Sergey;Pevzner, Pavel A.
通讯作者：
Pevzner, Pavel A.