A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy.
A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy.
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千万亿次自动成像管道，用于高通量透射电子显微镜映射神经元回路。
DOI：
10.1038/s41467-020-18659-3
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发表时间：
2020-10-02
影响因子：
16.6
通讯作者：
da Costa NM
中科院分区：
文献类型：
--
作者：
Yin W;Brittain D;Borseth J;Scott ME;Williams D;Perkins J;Own CS;Murfitt M;Torres RM;Kapner D;Mahalingam G;Bleckert A;Castelli D;Reid D;Lee WA;Graham BJ;Takeno M;Bumbarger DJ;Farrell C;Reid RC;da Costa NM
电子显微镜（EM）被广泛用于研究大脑中的细胞结构和网络连接。我们已经使用透射电子显微镜构建了一个并行成像管道，该管道可以扩展该技术，实现24/7连续自主成像，并能够获取千万亿次数据集。通过在不到6个月的时间内采集超过1 mm 3的小鼠新皮层，以突触分辨率跨越四个不同的视觉区域，证明了这种架构对大规模成像的适用性。对来自同一块的26，500多个组织切片进行了成像，产生了超过2 PB的数据集。流水线的组合突发采集速率为每秒3G像素，净速率为每秒600 Mpixel，其中六个显微镜并行运行。这项工作证明了在多个大脑区域和物种的皮层微电路的规模上获取EM数据集的可行性。电子显微镜（EM）是生物超微结构的黄金标准，但采集速度慢，不适合大体积。在这里，作者提出了一种并行成像管道，用于连续自主成像，具有六个传输EM，在不到6个月的时间内对1 mm 3的小鼠皮层进行成像。
Electron microscopy (EM) is widely used for studying cellular structure and network connectivity in the brain. We have built a parallel imaging pipeline using transmission electron microscopes that scales this technology, implements 24/7 continuous autonomous imaging, and enables the acquisition of petascale datasets. The suitability of this architecture for large-scale imaging was demonstrated by acquiring a volume of more than 1 mm3 of mouse neocortex, spanning four different visual areas at synaptic resolution, in less than 6 months. Over 26,500 ultrathin tissue sections from the same block were imaged, yielding a dataset of more than 2 petabytes. The combined burst acquisition rate of the pipeline is 3 Gpixel per sec and the net rate is 600 Mpixel per sec with six microscopes running in parallel. This work demonstrates the feasibility of acquiring EM datasets at the scale of cortical microcircuits in multiple brain regions and species. Electron microscopy (EM) is the gold standard for biological ultrastructure but acquisition speed is slow, making it unsuitable for large volumes. Here the authors present a parallel imaging pipeline for continuous autonomous imaging with six transmission EMs to image 1 mm3 of mouse cortex in less than 6 months.
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影响因子：
48
作者：
Hayworth, Kenneth J.;Xu, C. Shan;Lu, Zhiyuan;Knott, Graham W.;Fetter, Richard D.;Tapia, Juan Carlos;Lichtman, Jeff W.;Hess, Harald F.
通讯作者：
Hess, Harald F.
用于基于电子显微镜的连接组学的大批量En-Bloc染色。
影响因子：
16.6
作者：
Hua Y;Laserstein P;Helmstaedter M
通讯作者：
Helmstaedter M
影响因子：
64.8
作者：
Bock, Davi D.;Lee, Wei-Chung Allen;Kerlin, Aaron M.;Andermann, Mark L.;Hood, Greg;Wetzel, Arthur W.;Yurgenson, Sergey;Soucy, Edward R.;Kim, Hyon Suk;Reid, R. Clay
通讯作者：
Reid, R. Clay
影响因子：
64.8
作者：
Schmidt, Helene;Gour, Anjali;Helmstaedter, Moritz
通讯作者：
Helmstaedter, Moritz
影响因子：
64.5
作者：
Bumbarger, Daniel J.;Riebesell, Metta;Sommer, Ralf J.
通讯作者：
Sommer, Ralf J.