Differential Modulation of 25-hydroxycholecalciferol on Innate Immunity of Broiler Breeder Hens.
Differential Modulation of 25-hydroxycholecalciferol on Innate Immunity of Broiler Breeder Hens.
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DOI：
10.3390/ani11061742
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发表时间：
2021-06-10
期刊：
影响因子：
--
通讯作者：
Chen SE
中科院分区：
文献类型：
--
作者：
Chou PC;Lin PC;Wu SW;Wang CK;Chung TK;Walzem RL;Lai LS;Chen SE
尽管在炎症和呼吸爆发反应方面存在一些差异，但在肉种鸡中观察到R-与Ad-饲料摄入量对白细胞防御病原体的影响之间没有显著变化。总体而言，补充25-OH-D3对Ad-母鸡的先天免疫有更明显的影响。体外研究证实了25-OH-D3对挽救因葡萄糖和/或棕榈酸暴露而改变的免疫功能的不同作用。过去对肉鸡的免疫学研究主要集中在屠宰前快速生长期的幼鸡，可能无法反映成年鸡的免疫反应，特别是在长期限饲管理的种鸡中。该研究旨在评估在有和没有膳食25-羟基胆钙化醇（25-OH-D3）补充的情况下，种母鸡在R与自由采食量（Ad）饲料摄入量方面的先天免疫细胞功能。在51 - 68周龄的喂养试验中，Ad-饲料摄入始终抑制外周异嗜性粒细胞和/或单核细胞中的IL-1β分泌、呼吸爆发和细胞存活率。补充25-OH-D3主要抑制R-母鸡的IL-1β分泌和呼吸爆发，但促进Ad-母鸡的单核细胞吞噬、趋化和细菌杀伤活性，同时减轻高血糖、高脂血症和全身炎症。用来自R-母鸡的白细胞过夜培养物证实了25-OH-D3对挽救由葡萄糖和/或棕榈酸暴露改变的免疫功能的不同作用。使用特异性抑制剂的研究进一步证实了通过细胞类型和功能依赖性方式的糖脂毒性的作用机制。结果得出的结论是，尽管存在一些差异，但在R-饲料与Ad-饲料摄入量之间，白细胞对病原体的防御没有显著变化，但补充25-OH-D3在Ad-母鸡中发挥更显著的作用。
No predominant changes between R- vs. Ad-feed intake on leukocyte defense against pathogens were observed in broiler breeder hens despite some differences in inflammatory and respiratory burst responses. Overall, supplemental 25-OH-D3 had more pronounced effects on the innate immunity of Ad-hens. In vitro studies confirmed the differential effects of 25-OH-D3 to rescue immune functions altered by glucose and/or palmitic acid exposure. Past immunological studies in broilers focused on juveniles within the rapid pre-slaughter growth period and may not reflect adult immune responses, particularly in breeders managed with chronic feed restriction (R). The study aimed to assess innate immune cell functions in respect to R vs. ad libitum (Ad) feed intake in breeder hens with and without dietary 25-hydroxycholecalciferol (25-OH-D3) supplementation. Ad-feed intake consistently suppressed IL-1β secretion, respiratory burst, and cell livability in peripheral heterophils and/or monocytes along the feeding trial from the age of 51 to 68 weeks. Supplemental 25-OH-D3 repressed IL-1β secretion and respiratory burst of both cells mostly in R-hens, but promoted monocyte phagocytosis, chemotaxis, and bacterial killing activity in Ad-hens in accompany with relieved hyperglycemia, hyperlipidemia, and systemic inflammation. Overnight cultures with leukocytes from R-hens confirmed the differential effects of 25-OH-D3 to rescue immune functions altered by glucose and/or palmitic acid exposure. Studies with specific inhibitors further manifested the operative mechanisms via glucolipotoxicity in a cell type- and function-dependent manner. The results concluded no predominant changes between R- vs. Ad-feed intake on leukocyte defense against pathogens despite some differential differences, but supplemental 25-OH-D3 exerts more pronounced effects in Ad-hens.
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影响因子：
3
作者：
Lin, Hsuan-Yu;Chou, Pao-Chia;Chen, Shuen-Ei
通讯作者：
Chen, Shuen-Ei
影响因子：
--
作者：
Lenin R;Maria MS;Agrawal M;Balasubramanyam J;Mohan V;Balasubramanyam M
通讯作者：
Balasubramanyam M
影响因子：
1.8
作者：
Hala, K;Moore, C;Hofmann, A
通讯作者：
Hofmann, A
影响因子：
4.8
作者：
Hinkovska-Galcheva, V;Boxer, L;Shayman, JA
通讯作者：
Shayman, JA
影响因子：
4.1
作者：
Hewison M
通讯作者：
Hewison M