Mass spectrometry imaging of rat brain lipid profile changes over time following traumatic brain injury.
Mass spectrometry imaging of rat brain lipid profile changes over time following traumatic brain injury.
复制标题
DOI：
10.1016/j.jneumeth.2016.02.004
复制
发表时间：
2016-10-15
影响因子：
3
通讯作者：
Woods AS
中科院分区：
文献类型：
--
作者：
Roux A;Muller L;Jackson SN;Post J;Baldwin K;Hoffer B;Balaban CD;Barbacci D;Schultz JA;Gouty S;Cox BM;Woods AS
轻度创伤性脑损伤（TBI）是一种常见的公共卫生问题，可能导致慢性退行性疾病。膜脂作为细胞信号以及膜结构和细胞信号的组成部分，在组织对损伤的反应中起着关键作用。本研究证明了高分辨率质谱成像（MSI）在大鼠控制的脑震荡皮质冲击模型中评估脂质种类反应序列的能力。将植入的银纳米颗粒基质浅表植入大鼠脑切片，用于基质辅助激光解吸（MALDI）成像50 μm直径的微畴。离子迁移时间-飞行时间质谱（Ion-mobility time-of-flight MS）用于分析和绘制控制性皮质撞击（CCI）脑损伤后大鼠脑脂质组成随时间的变化。脑MS图像显示CCI部位附近鞘脂的变化，包括神经酰胺增加和鞘磷脂减少，并伴有甘油磷脂和胆固醇衍生物的变化。不同脂类的反应动力学不同；例如，神经酰胺在损伤后1天增加，而其他脂质变化发生在损伤后3至7天。银纳米颗粒MALDI基质是一种灵敏的新工具，用于揭示以前无法检测的细胞损伤反应和大脑神经、胶质和血管结构的重塑。脑外伤后的脂质生化和结构变化有助于突出可用于确定此类损伤严重程度以及评估潜在治疗效果的分子。
Mild traumatic brain injury (TBI) is a common public health issue that may contribute to chronic degenerative disorders. Membrane lipids play a key role in tissue responses to injury, both as cell signals and as components of membrane structure and cell signaling. This study demonstrates the ability of high resolution mass spectrometry imaging (MSI) to assess sequences of responses of lipid species in a rat controlled cortical impact model for concussion. A matrix of implanted silver nanoparticles was implanted superficially in brain sections for matrix-assisted laser desorption (MALDI) imaging of 50 μm diameter microdomains across unfixed cryostat sections of rat brain. Ion-mobility time-of-flight MS was used to analyze and map changes over time in brain lipid composition in a rats after Controlled Cortical Impact (CCI) TBI. Brain MS images showed changes in sphingolipids near the CCI site, including increased ceramides and decreased sphingomyelins, accompanied by changes in glycerophospholipids and cholesterol derivatives. The kinetics differed for each lipid class; for example ceramides increased as early as 1 day after the injury whereas other lipids changes occurred between 3 and 7 days post injury. Silver nanoparticles MALDI matrix is a sensitive new tool for revealing previously undetectable cellular injury response and remodeling in neural, glial and vascular structure of the brain. Lipid biochemical and structural changes after TBI could help highlighting molecules that can be used to determine the severity of such injuries as well as to evaluate the efficacy of potential treatments.
登录
查看更多内容
影响因子：
4.1
作者：
MARSHALL, LF;GAUTILLE, T;FOULKES, MA
通讯作者：
FOULKES, MA
影响因子：
3
作者：
Aureli, Massimo;Murdica, Valentina;Sonnino, Sandro
通讯作者：
Sonnino, Sandro
影响因子：
2.2
作者：
Górska, M;Dobrzyn, A;Namiot, Z
通讯作者：
Namiot, Z
影响因子：
2.1
作者：
Ionescu, Nicoleta;de Freitas, Caterina;Bueno, Allain A.
通讯作者：
Bueno, Allain A.
影响因子：
4.2
作者：
Levine, Brian;Fujiwara, Esther;Black, Sandra E.
通讯作者：
Black, Sandra E.