Dissemination of Genetic Acquisition/Loss Provides a Variety of Quorum Sensing Regulatory Properties in Pseudoalteromonas.
Dissemination of Genetic Acquisition/Loss Provides a Variety of Quorum Sensing Regulatory Properties in Pseudoalteromonas.
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遗传获得/丢失的传播为假交替单胞菌提供了多种群体感应调节特性
DOI：
10.3390/ijms19113636
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发表时间：
2018-11-18
影响因子：
5.6
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Yu Z;Ding Y;Yin J;Yu D;Zhang J;Zhang M;Ding M;Zhong W;Qiu J;Li J
群体感应（QS）使单细胞细菌能够通过化学信号进行交流，以同步群体水平的细菌行为。假交替单胞菌是在多种环境中发现的海洋细菌，其群体感应对栖息地适应的调控研究极其零碎。为了区分假交替单胞菌中群体感应调控所需的基因，运用了比较基因组学来确定12个分离株以及先前已测序基因组的泛基因组，并对基于酰基高丝氨酸内酯（AHL）的群体感应特性进行了表征。此外，利用转座子诱变来鉴定所选假交替单胞菌分离株中关键的群体感应调控基因。一个显著的特征是，假交替单胞菌诱导的生物传感器基于AHL的显色强度很可能与该属内群体感应调控基因的遗传异质性相关。对具有代表性的假交替单胞菌分离株中两个主要群体感应调控基因（luxO和rpoN）的相对表达水平的分析支持了这一观点。值得注意的是，假交替单胞菌中提出的综合群体感应调控模式和工作模型在系统发育上似乎包含了源自大肠杆菌、假单胞菌和弧菌的网络结构。通过转座子诱变绘制了几个相关基因。其中，一个编码右原点结合蛋白的基因（robp）被功能性鉴定为一个正向群体感应调控基因。该基因位于一个基因组不稳定区域，并且存在于上述通过生物信息学方法确定的群体感应调控模式中。所获得的数据强调，这种独特且分层组织的机制可能针对假交替单胞菌动态基因组中的群体感应关联，从而使细菌具有适应其适应性和生存优势的能力。
Quorum sensing (QS) enables single-celled bacteria to communicate with chemical signals in order to synchronize group-level bacterial behavior. Pseudoalteromonas are marine bacteria found in versatile environments, of which QS regulation for their habitat adaptation is extremely fragmentary. To distinguish genes required for QS regulation in Pseudoalteromonas, comparative genomics was deployed to define the pan-genomics for twelve isolates and previously-sequenced genomes, of which acyl-homoserine lactone (AHL)-based QS traits were characterized. Additionally, transposon mutagenesis was used to identify the essential QS regulatory genes in the selected Pseudoalteromonas isolate. A remarkable feature showed that AHL-based colorization intensity of biosensors induced by Pseudoalteromonas most likely correlates with QS regulators genetic heterogeneity within the genus. This is supported by the relative expression levels of two of the main QS regulatory genes (luxO and rpoN) analyzed in representative Pseudoalteromonas isolates. Notably, comprehensive QS regulatory schema and the working model proposed in Pseudoalteromonas seem to phylogenetically include the network architectures derived from Escherichia coli, Pseudomonas, and Vibrio. Several associated genes were mapped by transposon mutagenesis. Among them, a right origin-binding protein-encoding gene (robp) was functionally identified as a positive QS regulatory gene. This gene lies on a genomic instable region and exists in the aforementioned bioinformatically recruited QS regulatory schema. The obtained data emphasize that the distinctly- and hierarchically-organized mechanisms probably target QS association in Pseudoalteromonas dynamic genomes, thus leading to bacterial ability to accommodate their adaption fitness and survival advantages.
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影响因子：
14.9
作者：
Finn RD;Clements J;Arndt W;Miller BL;Wheeler TJ;Schreiber F;Bateman A;Eddy SR
通讯作者：
Eddy SR
影响因子：
3
作者：
Belarmino LC;Silva RL;Cavalcanti Nda M;Krezdorn N;Kido EA;Horres R;Winter P;Kahl G;Benko-Iseppon AM
通讯作者：
Benko-Iseppon AM
影响因子：
4.8
作者：
Bottomley, Matthew J.;Muraglia, Ester;Carfi, Andrea
通讯作者：
Carfi, Andrea
计算机和实验方法揭示了具有群体感应和芳香族生物降解系统的高度多样化的细菌——生物修复的潜在广泛应用
影响因子：
11.4
作者：
Huang, Yili;Zeng, Yanhua;Lin, Xiuchun
通讯作者：
Lin, Xiuchun
影响因子：
3.1
作者：
Jia, Qingmei;Lee, Bai-Yu;Horwitz, Marcus A.
通讯作者：
Horwitz, Marcus A.