ChipSeg: An Automatic Tool to Segment Bacterial and Mammalian Cells Cultured in Microfluidic Devices.
ChipSeg: An Automatic Tool to Segment Bacterial and Mammalian Cells Cultured in Microfluidic Devices.
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Chipseg:一种自动工具,用于分割细菌和哺乳动物细胞,并在微流体设备中培养。
DOI:
10.1021/acsomega.0c03906
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发表时间:
2021-02-02
期刊:
影响因子:
4.1
通讯作者:
Marucci L
中科院分区:
文献类型:
--
作者:
de Cesare I;Zamora-Chimal CG;Postiglione L;Khazim M;Pedone E;Shannon B;Fiore G;Perrino G;Napolitano S;di Bernardo D;Savery NJ;Grierson C;di Bernardo M;Marucci L
Extracting quantitative measurements from time-lapse images is necessary in external feedback control applications, where segmentation results are used to inform control algorithms. We describe ChipSeg, a computational tool that segments bacterial and mammalian cells cultured in microfluidic devices and imaged by time-lapse microscopy, which can be used also in the context of external feedback control. The method is based on thresholding and uses the same core functions for both cell types. It allows us to segment individual cells in high cell density microfluidic devices, to quantify fluorescent protein expression over a time-lapse experiment, and to track individual mammalian cells. ChipSeg enables robust segmentation in external feedback control experiments and can be easily customized for other experimental settings and research aims.
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影响因子:
3
作者:
Bajcsy P;Cardone A;Chalfoun J;Halter M;Juba D;Kociolek M;Majurski M;Peskin A;Simon C;Simon M;Vandecreme A;Brady M
通讯作者:
Brady M
影响因子:
3.8
作者:
Feng, Yuan;Dong, Fenglin;Mutic, Sasa
通讯作者:
Mutic, Sasa
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Ollion, Jean;Elez, Marina;Robert, Lydia
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Robert, Lydia
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Eliceiri, Kevin W.;Berthold, Michael R.;Goldberg, Ilya G.;Ibanez, Luis;Manjunath, B. S.;Martone, Maryann E.;Murphy, Robert F.;Peng, Hanchuan;Plant, Anne L.;Roysam, Badrinath;Stuurmann, Nico;Swedlow, Jason R.;Tomancak, Pavel;Carpenter, Anne E.
通讯作者:
Carpenter, Anne E.
影响因子:
23.9
作者:
Parchem, Ronald J.;Ye, Julia;Blelloch, Robert
通讯作者:
Blelloch, Robert